Evidence map›Paper›PMID 39013526›Full record

ArticleJournal of neuroscience methods2024

Using FASS-LTP in postmortem mice brain tissues to assess pathological synaptic function.

Joshua Currie, Shaneilahi Budhwani, Klarissa H Garza, Malav Mallipudi, Chandramouli Natarajan, Sravan Gopalkrishna Shetty Sreenivasamurthy, Balaji Krishnan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroscience methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Exploring the PLD1-tau interaction in Frontotemporal Dementia.bioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Joshua CurrieJohn Sealy School of Medicine, University of Texas Medical Branch at Galveston, USA; Mitchell Center for Neurodegenerative Diseases, Department of Neurology, University of Texas Medical Branch, USA.
Shaneilahi BudhwaniGraduate School for Biomedical Sciences, University of Texas Medical Branch, USA; Mitchell Center for Neurodegenerative Diseases, Department of Neurology, University of Texas Medical Branch, USA.
Klarissa H GarzaDepartment of Neurology, University of Texas Medical Branch, USA; Mitchell Center for Neurodegenerative Diseases, Department of Neurology, University of Texas Medical Branch, USA.
Malav MallipudiDepartment of Neurology, University of Texas Medical Branch, USA; School of Behavioral and Brain Sciences, University of Texas at Dallas, USA; Mitchell Center for Neurodegenerative Diseases, Department of Neurology, University of Texas Medical Branch, USA.
Chandramouli NatarajanGraduate School for Biomedical Sciences, University of Texas Medical Branch, USA.
Sravan Gopalkrishna Shetty SreenivasamurthyDepartment of Neurology, University of Texas Medical Branch, USA; Mitchell Center for Neurodegenerative Diseases, Department of Neurology, University of Texas Medical Branch, USA.
Balaji KrishnanJohn Sealy School of Medicine, University of Texas Medical Branch at Galveston, USA; Graduate School for Biomedical Sciences, University of Texas Medical Branch, USA; Department of Neurology, University of Texas Medical Branch, USA; Mitchell Center for Neurodegenerative Diseases, Department of Neurology, University of Texas Medical Branch, USA. Electronic address: bakrishn@utmb.edu.

Funding

Phospholipase D1 Mediated Early Events Affecting Synaptic Dysfunction in Alzheimer's Disease and Related DementiaR01AG063945 · NIA · UNIVERSITY OF TEXAS MED BR GALVESTON · PI KRISHNAN, BALAJI · 2020 to 2024
$2.0M
Predoctoral and Postdoctoral Training in Alzheimer's PathophysiologyT32AG067952 · NIA · UNIVERSITY OF TEXAS MED BR GALVESTON · PI KAYED, RAKEZ · 2021 to 2025
$1.5M
Synaptic PLD1 Levels/Signaling is Elevated Through Epigenetic Regulation in the CNS of AD Patients as a Function of Disease Severity and Cognitive DeclineR21AG059223 · NIA · UNIVERSITY OF TEXAS MED BR GALVESTON · PI KRISHNAN, BALAJI · 2018 to 2019
$434k
NIA NIH HHS R01 AG063945NIA NIH HHS R21 AG059223NIA NIH HHS T32 AG067952
6 · The paper itself

Abstract

backgroundStudy of synaptic integrity using conventional electrophysiology is a gold standard for quantitative assessment of neurodegeneration. Fluorescence assisted single-synapse long-term potentiation (FASS-LTP) provides a high throughput method to assess the synaptic integrity of neurotransmission within and between different brain regions as a measure of pharmacological efficacy in translational models. NEW

methodWe adapted the existing method to our purpose by adding a step during the thawing of frozen samples, by an extra step of placing them on a rocker at room temperature for 30 minutes immediately following thawing with constant mixing on a shaker. This allowed for gradual, uniform thawing, effectively separating the synaptosomes. Our study demonstrates FASS-LTP on four brain regions at 6- and 12-month periods in the 3xTg-AD mouse model, treating sibling cohorts with VU0155069 (a small molecule inhibitor) or vehicle (0.9 % saline).

resultsOur findings demonstrate the robust ability of the FASS-LTP technique to characterize the functional synaptic integrity maintained by disease-treatment therapies in multiple brain regions longitudinally using frozen brain tissue. COMPARISON WITH EXISTING

methodsBy providing a detailed, user-friendly protocol for this well-known analysis and including a recovery step improved the ability to robustly replicate the FASS-LTP between different brain regions. This may be extrapolated to a translational use on human clinical samples to improve understanding of the therapeutic impact on synaptic performance related to glutamate neurotransmission.

conclusionsFASS-LTP method offers a robust analysis of synaptosomes isolated from frozen tissue samples, demonstrating greater reproducibility in rodent and human synapses in physiological and pathological states.

Indexed as

BrainLong-Term PotentiationSynapsesAnimalsDisease Models, AnimalFemaleMaleMiceMice, Inbred C57BLMice, TransgenicSynaptosomesFASS-LTPFlow cytometryFrozen brain tissuesGlutamatergic synapseHigh throughputSynaptic neurotransmission

Identifiers

PMID39013526
PMCPMC12940509

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.