Evidence mapPaperPMID 39017751Full record

ArticleIn vitro cellular & developmental biology. Animal2024

SFRP1 reduces neutrophil infiltration and inhibits the Wnt/β-catenin pathway to alleviate oral submucous fibrosis.

Rong Zhou, Lin Yin, Xin Zhang, Keke Zhu

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Article in In vitro cellular & developmental biology. Animal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Rong ZhouDepartment of Stomatology, Changsha Hospital for Maternal and Child Health Care, Changsha, China.
Lin YinDepartment of Stomatology, Changsha Hospital for Maternal and Child Health Care, Changsha, China.
Xin ZhangDepartment of Stomatology, Changsha Hospital for Maternal and Child Health Care, Changsha, China.
Keke ZhuDepartment of Stomatology, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, 410208, Hunan, China. Zhukeke_HNUCM@163.com.ORCID http://orcid.org/0009-0007-6129-0039

Funding

712 Talent Project supported by Changsha Maternal and Child Health Hospital 20211159Hunan Provincial Administration of Traditional Chinese Medicine Scientific Research Project B2024068Research Project supported by Hunan Provincial Department of Education 22C0190
6 · The paper itself

Abstract

Oral submucous fibrosis (OSF) is a precancerous condition characterized by oral mucosal atrophy with fibrosis of the submucosal tissue. OSF has a high prevalence, and treatment requires improvement. Our study aims to investigate the role and mechanism of secreted frizzled-related protein 1 (SFRP1) in OSF. We constructed an arecoline-induced OSF mice model. Through Pearson's correlation analysis, we investigated the association between SFRP1 levels and expressions of proteins related to the Wnt/β-catenin signaling pathway, as well as the correlation between SFRP1 levels and the degree of neutrophil infiltration. Moreover, neutrophil infiltration intensity, tissue fibrosis degree, and levels of β-catenin, Cyclin D1, and c-myc were evaluated after SFRP1 overexpression treatment through immunohistochemical and biochemical assays. A Wnt/β-catenin pathway activator was used to investigate the molecular mechanism of SFRP1 in the arecoline-induced OSF cell model. Compared with the control group, mice in the OSF group exhibited increased collagen deposition and more severe fibrosis in the oral mucosal tissue (OMT). In the OMT of OSF mice, the levels of SFRP1 were decreased. The levels of SFRP1 exhibited a negative correlation with the levels of Wnt/β-catenin proteins and neutrophil infiltration in the OMT. Upon SFRP1 overexpression, there was a reduction in neutrophil infiltration and fibrosis in the OMT, as well as inhibition of Wnt/β-catenin-related proteins. In vitro, the Wnt/β-catenin pathway activator further reversed the effect of SFRP1 overexpression on OSF. SFRP1 attenuates OSF by reducing neutrophil infiltration and inhibiting the Wnt/β-catenin pathway.

Indexed as

beta CateninNeutrophil InfiltrationOral Submucous FibrosisWnt Signaling PathwayAnimalsArecolineDisease Models, AnimalHumansIntercellular Signaling Peptides and ProteinsMaleMembrane ProteinsMiceMice, Inbred C57BLMouth MucosaProto-Oncogene Proteins c-mycSecreted Frizzled-Related ProteinsArecolinebeta CateninIntercellular Signaling Peptides and ProteinsMembrane ProteinsProto-Oncogene Proteins c-mycSecreted Frizzled-Related ProteinsSfrp1 protein, mouseNeutrophil infiltrationOSFSFRP1Wnt/β-catenin

Identifiers

PMID39017751

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.