Evidence map›Paper›PMID 39017882›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Ultrasound-Guided Fine Needle Biopsy of Human Axillary Lymph Nodes to Assess B Cell Responses to Vaccination.

Alexandra Carey Hoppé, Solange Obeid, Brad Milner, Vanessa Venturi, C Mee Ling Munier

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Alexandra Carey HoppéImmunology and Pathogenesis Program, Kirby Institute, UNSW Sydney, Sydney, NSW, Australia.
Solange ObeidSt Vincent's Hospital Sydney, Sydney, NSW, Australia.
Brad MilnerSt Vincent's Hospital Sydney, Sydney, NSW, Australia.
Vanessa Venturi *Immunology and Pathogenesis Program, Kirby Institute, UNSW Sydney, Sydney, NSW, Australia.
C Mee Ling Munier *Immunology and Pathogenesis Program, Kirby Institute, UNSW Sydney, Sydney, NSW, Australia. cmunier@kirby.unsw.edu.au.

Funding

National Health and Medical Research Council 2021/GNT2012863
6 · The paper itself

Abstract

Ultrasound-guided fine needle biopsy, also known as fine needle aspiration, of human axillary lymph nodes is a safe and effective procedure to assess the immune response within the lymph nodes following vaccination. Once acquired, lymph node cells can be characterized via flow cytometric immunophenotyping and/or single-cell RNA sequencing for gene expression and T and B cell receptors. Analysis of the immune cells from the lymph nodes enables the investigation of T and B cells that may interact at this site. These interactions may lead to germinal center formation and expansion, critical for the generation of effective immunity to vaccination. Directly studying the dynamic processes and interaction of the key cells has been challenging in humans due to the anatomically protected location of these cells. Here, we describe the methods involved in ultrasound-guided fine needle biopsy of human axillary lymph nodes in response to vaccination and subsequent analyses of the B cell populations.

Indexed as

AxillaB-LymphocytesLymph NodesVaccinationBiopsy, Fine-NeedleFlow CytometryHumansImage-Guided BiopsyImmunophenotypingB cell receptor (BCR)B cellsFine needle aspirate (FNA)Fine needle biopsy (FNB)HumanImmune responseLymph nodesVaccination

Identifiers

PMID39017882

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.