Evidence map›Paper›PMID 39020017›Full record

ArticleScientific reports2024

The role of lipid peroxidation in epithelial-mesenchymal transition of retinal pigment epithelial cells.

Wang You, Kunihiro Azuma, Toshiro Iwagawa, Sumiko Watanabe, Makoto Aihara, Tomoyasu Shiraya, Takashi Ueta

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Wang YouDepartment of Ophthalmology, The Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo, Tokyo, Japan.
Kunihiro AzumaDepartment of Ophthalmology, The Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo, Tokyo, Japan.
Toshiro IwagawaDepartment of Ophthalmology, The Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo, Tokyo, Japan.
Sumiko WatanabeDepartment of Ophthalmology, The Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo, Tokyo, Japan.
Makoto AiharaDepartment of Ophthalmology, The Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo, Tokyo, Japan.
Tomoyasu ShirayaDepartment of Ophthalmology, The Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo, Tokyo, Japan.
Takashi UetaDepartment of Ophthalmology, The Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo, Tokyo, Japan. ueta-tky@umin.ac.jp.

Funding

Japan Society for the Promotion of Science 19K18872Japan Society for the Promotion of Science 21K09758
6 · The paper itself

Abstract

Epithelial-Mesenchymal Transition (EMT) of retinal pigment epithelial (RPE) cells is recognized as pivotal in various retinal diseases. Previous studies have suggested a reciprocal regulation between reactive oxygen species (ROS) and EMT, though the involvement of peroxidized lipids or the effects of reducing them has remained unclear. The present study disclosed that EMT of ARPE-19 cells induced by TGF-β2 and TNF-α involves increased lipid peroxidation, and Ferrostatin-1 (Fer-1), a lipophilic antioxidative agent, successfully inhibited the increase in lipid peroxidation. Fer-1 suppressed the formation of EMT-associated fibrotic deposits, while EMT induction or Fer-1 treatment did not influence the cell viability or proliferation. Functionally, Fer-1 impeded EMT-driven cell migration and reduction in transepithelial electrical resistance. It demonstrated regulatory prowess by downregulating the mesenchymal marker fibronectin, upregulating the epithelial marker ZO-1, and inhibiting the EMT-associated transcriptional factor ZEB1. Additionally, VEGF, a major pathogenic cytokine in various retinal diseases, is also upregulated during EMT, and Fer-1 significantly mitigated the effect. The present study disclosed the involvement of lipid peroxidation in EMT of RPE cells, and suggests the suppression of lipid peroxidation may be a potential therapeutic target in retinal diseases in which EMT is implicated.

Indexed as

Epithelial-Mesenchymal TransitionLipid PeroxidationRetinal Pigment EpitheliumCell LineCell MovementCell ProliferationCell SurvivalEpithelial CellsFibronectinsHumansReactive Oxygen SpeciesTransforming Growth Factor beta2Tumor Necrosis Factor-alphaVascular Endothelial Growth Factor AZinc Finger E-box-Binding Homeobox 1Zonula Occludens-1 ProteinFibronectinsReactive Oxygen SpeciesTransforming Growth Factor beta2Tumor Necrosis Factor-alphaVascular Endothelial Growth Factor AZEB1 protein, humanZinc Finger E-box-Binding Homeobox 1Zonula Occludens-1 ProteinARPE-19Epithelial–mesenchymal transitionFerrostatin-1Lipid peroxidationRetinal pigment epithelium

Identifiers

PMID39020017
PMCPMC11255318

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.