SynthesisJournal of experimental botany2024
Greater aperture counteracts effects of reduced stomatal density on water use efficiency: a case study on sugarcane and meta-analysis.
Synthesis in Journal of experimental botany, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Water use efficiency in cereal crops: stomatal, hydraulic, biochemical mechanisms, and translational strategies for improvement.Photosynthesis research · 2026Review
- High-resolution microCT reveals relationships between stomata and interior leaf anatomy in sorghum.Plant physiology · 2026Article
- Brachypodium SPEECHLESS2 Promoter Drives Expression of a Synthetic EPF to Reduce Stomatal Density in Sugarcane Without Pleiotropic Effects.Plant biotechnology journal · 2026Article
- Virus-induced gene editing of stomatal regulators in Nicotiana benthamiana enables rapid functional genomics.The Plant journal : for cell and molecular biology · 2026Article
- Stomata in-sight: Integrating live confocal microscopy with leaf gas exchange and environmental control.Plant physiology · 2025Article
- SCAN: an automated phenotyping tool for real-time capture of leaf stomatal traits in canola.Journal of experimental botany · 2025Article
- Article
- Reduced stomatal density in wheat overexpressing EPIDERMAL PATTERNING FACTOR1 differentially affects red and blue light responses.Plant physiology · 2025Article
- Induction of autotetraploid and morphological, anatomical, and cytological studies of Trichosanthes cucumerina Linn.BMC plant biology · 2025Article
- Reduced stomatal density improves water-use efficiency in grapevine under climate scenarios of decreased water availability.Plant cell reports · 2025Article
- Stomata-Photosynthesis Synergy Mediates Combined Heat and Salt Stress Tolerance in Sugarcane Mutant M4209.Plant, cell & environment · 2025Article
- Impact of climate-driven changes in temperature on stomatal anatomy and physiology.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- The combined effect of decreased stomatal density and aperture increases water use efficiency in maize.Scientific reports · 2025Article
- Polyploidy drives changes in tissue allocation modifying whole-plant water relations.Plant physiology · 2025Article
- Machine learning-enabled computer vision for plant phenotyping: a primer on AI/ML and a case study on stomatal patterning.Journal of experimental botany · 2024Review
- Reducing stomatal density by expression of a synthetic epidermal patterning factor increases leaf intrinsic water use efficiency and reduces plant water use in a C4 crop.Journal of experimental botany · 2024Article
- Stomata: custodians of leaf gaseous exchange.Journal of experimental botany · 2024Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Stomata regulate CO2 and water vapor exchange between leaves and the atmosphere. Stomata are a target for engineering to improve crop intrinsic water use efficiency (iWUE). One example is by expressing genes that lower stomatal density (SD) and reduce stomatal conductance (gsw). However, the quantitative relationship between reduced SD, gsw, and the mechanisms underlying it is poorly understood. We addressed this knowledge gap using low-SD sugarcane (Saccharum spp. hybrid) as a case study alongside a meta-analysis of data from 10 species. Transgenic expression of EPIDERMAL PATTERNING FACTOR 2 from Sorghum bicolor (SbEPF2) in sugarcane reduced SD by 26-38% but did not affect gsw compared with the wild type. Further, no changes occurred in stomatal complex size or proxies for photosynthetic capacity. Measurements of gas exchange at low CO2 concentrations that promote complete stomatal opening to normalize aperture size between genotypes were combined with modeling of maximum gsw from anatomical data. These data suggest that increased stomatal aperture is the only possible explanation for maintaining gsw when SD is reduced. Meta-analysis across C3 dicots, C3 monocots, and C4 monocots revealed that engineered reductions in SD are strongly correlated with lower gsw (r2=0.60-0.98), but this response is damped relative to the change in anatomy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.