ArticleJournal of experimental botany2024
Reducing stomatal density by expression of a synthetic epidermal patterning factor increases leaf intrinsic water use efficiency and reduces plant water use in a C4 crop.
Article in Journal of experimental botany, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Greater aperture counteracts effects of reduced stomatal density on water use efficiency: a case study on sugarcane and meta-analysis.Journal of experimental botany · 2024Pooled it
- Candidate genes with antagonistic roles in stomatal development are associated with population-wide variation in apple.Horticulture research · 2026Article
- Water use efficiency in cereal crops: stomatal, hydraulic, biochemical mechanisms, and translational strategies for improvement.Photosynthesis research · 2026Review
- High-resolution microCT reveals relationships between stomata and interior leaf anatomy in sorghum.Plant physiology · 2026Article
- Coordinated leaf anatomy buffers the effects of reduced stomatal density on CO2 diffusion.Plant physiology · 2026Article
- Brachypodium SPEECHLESS2 Promoter Drives Expression of a Synthetic EPF to Reduce Stomatal Density in Sugarcane Without Pleiotropic Effects.Plant biotechnology journal · 2026Article
- Virus-induced gene editing of stomatal regulators in Nicotiana benthamiana enables rapid functional genomics.The Plant journal : for cell and molecular biology · 2026Article
- Generalizability and transferability of machine learning models using hyperspectral reflectance data for maize traits.Scientific reports · 2026Article
- Biotechnological and genetic innovations to enhance sorghum adaptation under climate change.Frontiers in plant science · 2026Review
- Stomata in-sight: Integrating live confocal microscopy with leaf gas exchange and environmental control.Plant physiology · 2025Article
- Plant hydraulic traits influencing crop production in water-limited environments.Plant physiology · 2025Review
- Physiological and transcriptomic analyses reveal the mechanisms underlying leaf color variation in zebra-striped mutants of oat (Avena sativa).BMC plant biology · 2025Article
- Reduced stomatal density improves water-use efficiency in grapevine under climate scenarios of decreased water availability.Plant cell reports · 2025Article
- Translational insights into abiotic interactions: From Arabidopsis to crop plants.The Plant cell · 2025Review
- Stomata-Photosynthesis Synergy Mediates Combined Heat and Salt Stress Tolerance in Sugarcane Mutant M4209.Plant, cell & environment · 2025Article
- Impact of climate-driven changes in temperature on stomatal anatomy and physiology.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Needs and opportunities to future-proof crops and the use of crop systems to mitigate atmospheric change.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Challenges of translating Arabidopsis insights into crops.The Plant cell · 2025Review
- The combined effect of decreased stomatal density and aperture increases water use efficiency in maize.Scientific reports · 2025Article
- Machine learning-enabled computer vision for plant phenotyping: a primer on AI/ML and a case study on stomatal patterning.Journal of experimental botany · 2024Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Enhancing crop water use efficiency (WUE) is a key target trait for climatic resilience and expanding cultivation on marginal lands. Engineering lower stomatal density to reduce stomatal conductance (gs) has improved WUE in multiple C3 crop species. However, reducing gs in C3 species often reduces photosynthetic carbon gain. A different response is expected in C4 plants because they possess specialized anatomy and biochemistry which concentrates CO2 at the site of fixation. This modifies the relationship of photosynthesis (AN) with intracellular CO2 concentration (ci), such that photosynthesis is CO2 saturated and reductions in gs are unlikely to limit AN. To test this hypothesis, genetic strategies were investigated to reduce stomatal density in the C4 crop sorghum. Constitutive expression of a synthetic epidermal patterning factor (EPF) transgenic allele in sorghum led to reduced stomatal densities, reduced gs, reduced plant water use, and avoidance of stress during a period of water deprivation. In addition, moderate reduction in stomatal density did not increase stomatal limitation to AN. However, these positive outcomes were associated with negative pleiotropic effects on reproductive development and photosynthetic capacity. Avoiding pleiotropy by targeting expression of the transgene to specific tissues could provide a pathway to improved agronomic outcomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.