Evidence map›Paper›PMID 39043179›Full record

ArticleCell2024

Presynaptic sensor and silencer of peptidergic transmission reveal neuropeptides as primary transmitters in pontine fear circuit.

Dong-Il Kim, Sekun Park, Seahyung Park, Mao Ye, Jane Y Chen, Sukjae J Kang, Jinho Jhang, Avery C Hunker, Larry S Zweifel, Kathleen M Caron and 4 more

Abstract read
In one paragraph

Article in Cell, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Review
  7. Article
  8. ParabrachialeNeuro · 2025
    Article
  9. Article
  10. Nociceptors use multiple neurotransmitters to drive pain.bioRxiv : the preprint server for biology · 2025
    Article
  11. Article
  12. Mechanisms of glucagon-like-peptide 1 in the brain beyond metabolic effects.Annals of pediatric endocrinology & metabolism · 2025
    Article
  13. Article
  14. Review
  15. Review
  16. Review
  17. Article
  18. Article
  19. Sex Differences in Central Amygdala Glutamate Responses to Calcitonin Gene-Related Peptide.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025
    Article
  20. Pain persists in mice lacking both Substance P and CGRPα signaling.bioRxiv : the preprint server for biology · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Dong-Il KimPeptide Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Sekun ParkHoward Hughes Medical Institute, Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
Seahyung ParkPeptide Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Mao YePeptide Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Jane Y ChenHoward Hughes Medical Institute, Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
Sukjae J KangPeptide Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Jinho JhangPeptide Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Avery C HunkerDepartment of Pharmacology, University of Washington, Seattle, WA 98195, USA.
Larry S ZweifelDepartment of Pharmacology, University of Washington, Seattle, WA 98195, USA.
Kathleen M CaronDepartment of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Joan M VaughanPeptide Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Alan SaghatelianPeptide Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Richard D PalmiterHoward Hughes Medical Institute, Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
Sung HanPeptide Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA; Center for Neuroscience Imaging Research, Institute for Basic Science, Suwon 16419, Republic of Korea; Department of Biomedical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea. Electronic address: sunghan@salk.edu.

Funding

University of Washington Center of Excellence in Opioid Addiction ResearchP30DA048736 · NIDA · UNIVERSITY OF WASHINGTON · PI Charles Chavkin · 2019 to 2026
$13.0M
Contribution of the Parabrachial CGRP-Expressing Neurons to the Pathophysiology of Panic DisorderR01MH116203 · NIMH · SALK INSTITUTE FOR BIOLOGICAL STUDIES · PI HAN, SUNG · 2018 to 2022
$3.0M
Monitoring presynaptic release of neuropeptides in awake behaving animalsRF1NS128680 · NINDS · SALK INSTITUTE FOR BIOLOGICAL STUDIES · PI HAN, SUNG · 2022 to 2022
$2.0M
NIDA NIH HHS P30 DA048736NIMH NIH HHS R01 MH116203NINDS NIH HHS RF1 NS128680
6 · The paper itself

Abstract

Neurons produce and release neuropeptides to communicate with one another. Despite their importance in brain function, circuit-based mechanisms of peptidergic transmission are poorly understood, primarily due to the lack of tools for monitoring and manipulating neuropeptide release in vivo. Here, we report the development of two genetically encoded tools for investigating peptidergic transmission in behaving mice: a genetically encoded large dense core vesicle (LDCV) sensor that detects presynaptic neuropeptide release and a genetically encoded silencer that specifically degrades neuropeptides inside LDCVs. Using these tools, we show that neuropeptides, not glutamate, encode the unconditioned stimulus in the parabrachial-to-amygdalar threat pathway during Pavlovian threat learning. We also show that neuropeptides play important roles in encoding positive valence and suppressing conditioned threat response in the amygdala-to-parabrachial endogenous opioidergic circuit. These results show that our sensor and silencer for presynaptic peptidergic transmission are reliable tools to investigate neuropeptidergic systems in awake, behaving animals.

Indexed as

FearNeuropeptidesAmygdalaAnimalsConditioning, ClassicalMaleMiceMice, Inbred C57BLNeuronsNeurotransmitter AgentsPonsPresynaptic TerminalsSynaptic TransmissionNeuropeptidesNeurotransmitter Agents

Identifiers

PMID39043179
PMCPMC11380597

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.