Evidence map›Paper›PMID 39046255›Full record

ArticleJournal of clinical microbiology2024

Performance of the cobas EBV and cobas BKV assays: multi-site comparison of standardized quantitation.

Laura Mannonen, Pia Jokela, Marianne Kragh Thomsen, Sabine Yerly, Gustavo Cilla, Daniel Jarem, Jesse A Canchola, Mark Hopkins

Abstract readComparative StudyMulticenter StudyEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Laura MannonenDepartment of Clinical Microbiology, HUS Diagnostic Center, HUSLAB, Clinical Microbiology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Pia JokelaDepartment of Clinical Microbiology, HUS Diagnostic Center, HUSLAB, Clinical Microbiology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Marianne Kragh ThomsenDepartment of Clinical Microbiology, Aarhus University Hospital, Aarhus, Denmark.
Sabine YerlyLaboratory of Virology, Diagnostic Department, Geneva University Hospitals, Geneva, Switzerland.
Gustavo CillaDepartment of Microbiology, Donostia University Hospital and Biodonostia Health Research Institute, San Sebastián, Spain.
Daniel JaremClinical Development and Medical Affairs, Roche Molecular Systems, Inc., Pleasanton, California, USA.
Jesse A CancholaCDMA Biometrics, Biostatistics Group, Roche Molecular Systems, Inc., Pleasanton, California, USA.
Mark HopkinsDepartment of Virology, Barts Health NHS Trust, London, United Kingdom.ORCID 0000-0003-4744-8542

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Guidelines recommend monitoring of Epstein-Barr virus (EBV) and BK virus (BKV) in solid organ and hematopoietic stem cell transplant patients. The majority of quantitative DNA testing for EBV and BKV employs unstandardized individual laboratory-developed testing solutions (LDTs), with implications for accuracy, reproducibility, and comparability between laboratories. The performance of the cobas EBV and cobas BKV assays was assessed across five laboratories, using the World Health Organization International Standards (WHO IS) for EBV and BKV, and the National Institute of Standards and Technology Quantitative Standard for BKV, and results were compared with the LDTs in use at the time. Methods were also compared using locally sourced clinical specimens. Variation was high when laboratories reported EBV or BKV DNA values using LDTs, where quantitative values were observed to differ by up to 1.5 log

Indexed as

BK VirusEpstein-Barr Virus InfectionsHerpesvirus 4, HumanViral LoadDNA, ViralHumansMolecular Diagnostic TechniquesPolyomavirus InfectionsReproducibility of ResultsTumor Virus InfectionsDNA, Viralassay performanceassay standardizationassay variabilityBKVEpstein-Barr viruslaboratory-developed testpost-transplant monitoringviral load quantitation

Identifiers

PMID39046255
PMCPMC11323559

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.