Evidence map›Paper›PMID 39060918›Full record

ArticleMicrobial cell factories2024

Bioprocessing of camptothecin from Alternaria brassicicola, an endophyte of Catharanthus roseus, with a strong antiproliferative activity and inhibition to Topoisomerases.

Nouran A A Abd El-Hady, Abdelaleim I ElSayed, Khalid M Wadan, Sayed S El-Saadany, Ashraf S A El-Sayed

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Genomic and Metabolomic Analysis of the Endophytic FungusAntibiotics (Basel, Switzerland) · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Nouran A A Abd El-HadyEnzymology and Fungal Biotechnology Lab, Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt.
Abdelaleim I ElSayedBiochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig, 44519, Egypt.
Khalid M WadanBiochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig, 44519, Egypt.
Sayed S El-SaadanyBiochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig, 44519, Egypt.
Ashraf S A El-SayedEnzymology and Fungal Biotechnology Lab, Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt. ash.elsayed@gmail.com.

Funding

Academy of Scientific Research and Technology ASRT-2022
6 · The paper itself

Abstract

Suppression of fungal camptothecin (CPT) biosynthesis with the preservation and successive subculturing is the challenge that impedes fungi from the industrial application, so, screening for a novel fungal isolate with a conceivable stable producing potency of CPT was the main objective of this work. Catharanthus roseus with diverse contents of bioactive metabolites could have a plethora of novel endophytes with unique metabolic properties. Among the endophytes of C. roseus, Alternaria brassicicola EFBL-NV OR131587.1 was the highest CPT producer (96.5 μg/L). The structural identity of the putative CPT was verified by HPLC, FTIR, HNMR and LC-MS/MS, with a molecular mass 349 m/z, and molecular fragmentation patterns that typically identical to the authentic one. The purified A. brassicicola CPT has a strong antiproliferative activity towards UO-31 (0.75 μM) and MCF7 (3.2 μM), with selectivity index 30.8, and 7.1, respectively, in addition to resilient activity to inhibit Topo II (IC

Indexed as

AlternariaCamptothecinCatharanthusCell ProliferationEndophytesAntineoplastic AgentsApoptosisCell Line, TumorHumansMCF-7 CellsTopoisomerase I InhibitorsAntineoplastic AgentsCamptothecinTopoisomerase I InhibitorsAlternaria brassicicolaAnticancer activityApoptosisCamptothecinCatharanthus roseusCell cycleTopoisomerase inhibitors

Identifiers

PMID39060918
PMCPMC11282713

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.