Evidence map›Paper›PMID 39062012›Full record

ArticleBiomedicines2024

Suppressing Pro-Apoptotic Proteins by siRNA in Corneal Endothelial Cells Protects against Cell Death.

Susanne Staehlke, Siddharth Mahajan, Daniel Thieme, Peter Trosan, Thomas A Fuchsluger

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedicines, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Susanne StaehlkeDepartment of Ophthalmology, Rostock University Medical Center, 18057 Rostock, Germany.ORCID 0000-0002-0251-8263
Siddharth MahajanDepartment of Ophthalmology, University of Erlangen-Nuremberg, 91054 Erlangen, Germany.
Daniel ThiemeDepartment of Ophthalmology, University of Erlangen-Nuremberg, 91054 Erlangen, Germany.
Peter TrosanDepartment of Ophthalmology, Rostock University Medical Center, 18057 Rostock, Germany.
Thomas A FuchslugerDepartment of Ophthalmology, Rostock University Medical Center, 18057 Rostock, Germany.

Funding

Rostock University Medical Center, FORUN program 889039
6 · The paper itself

Abstract

Corneal endothelial cells (CE) are critical for the cornea's transparency. For severe corneal damage, corneal tissue transplantation is the most promising option for restoring vision. However, CE apoptotic cell death occurs during the storage of donor corneas for transplantation. This study used small interfering (si)RNA-mediated silencing of pro-apoptotic proteins as a novel strategy to protect CE against apoptosis. Therefore, the pro-apoptotic proteins Bax and Bak were silenced in the human corneal endothelial cell line (HCEC-12) by transfection with Accell™siRNA without any adverse effects on cell viability. When apoptosis was induced, e.g., etoposide, the caspase-3 activity and Annexin V-FITC/PI assay indicated a significantly reduced apoptosis rate in Bax+Bak-siRNA transfected HCECs compared to control (

Indexed as

Annexin Vanti-apoptotic Bax and Bakapoptosiscaspase-3confocal laser scanning microscopycorneal endothelial cellsdonor corneasiRNATUNEL

Identifiers

PMID39062012
PMCPMC11274739

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.