Evidence map›Paper›PMID 39071681›Full record

ArticleHeliyon2024

Molecular analysis of human tick-bitten skin yields signatures associated with distinct spatial and temporal trajectories - A proof-of-concept study.

Wenna Lee, Rym Ben-Othman, Patrycja Skut, Amy Huey-Yi Lee, Amanda D Barbosa, Miles Beaman, Andrew Currie, Nathan T Harvey, Prasad Kumarasinghe, Roy A Hall and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Heliyon, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. The BCGMethodsX · 2025
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Wenna LeeCentre for Biosecurity and One Health, Harry Butler Institute, Murdoch University, Murdoch, WA, Australia.
Rym Ben-OthmanTelethon Kids Institute, Perth, WA, Australia.
Patrycja SkutTelethon Kids Institute, Perth, WA, Australia.
Amy Huey-Yi LeeMolecular Biology and Biochemistry, Simon Fraser University, British Columbia, Canada.
Amanda D BarbosaCentre for Biosecurity and One Health, Harry Butler Institute, Murdoch University, Murdoch, WA, Australia.
Miles BeamanFaculty of Health and Medical Sciences, Pathology & Laboratory Medicine, University of Western Australia, Perth, WA, Australia.
Andrew CurrieSchool of Medical, Molecular, and Forensic Sciences, College of Environmental and Life Sciences, Murdoch University, Murdoch, WA, Australia.
Nathan T HarveyFaculty of Health and Medical Sciences, Pathology & Laboratory Medicine, University of Western Australia, Perth, WA, Australia.
Prasad KumarasingheSchool of Medicine, University of Western Australia, Crawley, WA, Australia.
Roy A HallAustralian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, Australia.
James PotterAustralian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, Australia.
Stephen GravesAustralian Rickettsial Reference Laboratory, Barwon Health, Geelong, VIC, Australia.
Nicholas P WestSchool of Pharmacy and Medical Sciences, and Menzies Health Institute, Griffith University, QLD, Australia.
Amanda J CoxSchool of Pharmacy and Medical Sciences, and Menzies Health Institute, Griffith University, QLD, Australia.
Peter J IrwinSchool of Veterinary Medicine, College of Environmental and Life Sciences, Murdoch University, Murdoch, WA, Australia.
Tobias R KollmannTelethon Kids Institute, Perth, WA, Australia.
Charlotte L OskamCentre for Biosecurity and One Health, Harry Butler Institute, Murdoch University, Murdoch, WA, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tick-associated diseases present challenges due to tridirectional interactions among host-specific responses, tick toxins and salivary proteins as well as microbes. We aimed to uncover molecular mechanisms in tick-bitten skin samples (cases) and contralateral skin samples (controls) collected simultaneously from the same participants, using spatial transcriptomics. Cases and controls analysed using NanoString GeoMx Digital Spatial Profiler identified 274 upregulated and 840 downregulated differentially expressed genes (DEGs), revealing perturbations in keratinization and immune system regulation. Samples of skin biopsies taken within 72 h post tick-bite DEGs had changes in protein metabolism and viral infection pathways as compared to samples taken 3 months post tick-bite, which instead displayed significant perturbations in several epigenetic regulatory pathways, highlighting the temporal nature of the host response following tick bites. Within-individual signatures distinguished tick-bitten samples from controls and identified between-individual signatures, offering promise for future biomarker discovery to guide prognosis and therapy.

Indexed as

Emerging diseasesSpatial profilingSpatial transcriptomicsTick-borne diseasesTick-host-pathogen

Identifiers

PMID39071681
PMCPMC11283101

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.