Evidence mapPaperPMID 39080613Full record

ArticleBMC cancer2024

HOXC11-mediated regulation of mitochondrial function modulates chemoresistance in colorectal cancer.

Shicheng Chu, Xiang Ren, Lianmeng Cao, Chong Ma, Kai Wang

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In one paragraph

Article in BMC cancer, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Shicheng Chu *Department of Colorectal Hernia Surgery, Binzhou Medical University Hospital, Binzhou, Shandong, China.
Xiang Ren *Department of Colorectal Hernia Surgery, Binzhou Medical University Hospital, Binzhou, Shandong, China.
Lianmeng CaoDepartment of Gastrointestinal Surgery, Binzhou Medical University Hospital, Binzhou, Shandong, China.
Chong MaDepartment of Colorectal Hernia Surgery, Binzhou Medical University Hospital, Binzhou, Shandong, China. byfymc@126.com.
Kai WangDepartment of Colorectal Hernia Surgery, Binzhou Medical University Hospital, Binzhou, Shandong, China. byfyjzcswk@126.com.

Funding

The Scientific Research Program of Binzhou Medical University BY2020KJ08
6 · The paper itself

Abstract

backgroundChemoresistance remains a significant challenge in colorectal cancer (CRC) treatment, necessitating a deeper understanding of its underlying mechanisms. HOXC11 has emerged as a potential regulator in various cancers, but its role in CRC chemoresistance remains unclear.

methodsSulforhodamine B assay was employed to assess the cell viability of CRC cells following treatment with chemotherapeutic drugs. Immunofluorescence staining was performed to examine the subcellular localization of HOXC11 in normal and chemoresistant CRC cells. The Seahorse mito stress test was conducted to evaluate the mitochondrial respiratory function of CRC cells. Real-time PCR was utilized to measure the expression level and copy number of mitochondrial DNA (mtDNA).

resultsOur findings revealed that HOXC11 was overexpressed in CRC cells compared to normal colorectal cells and correlated with poorer prognosis in CRC patients. Knockout of HOXC11 reversed acquired chemoresistance in CRC cells. Furthermore, we observed a functional subset of HOXC11 localized to the mitochondria in chemoresistant CRC cells, which regulated mitochondrial function by modulating mtDNA transcription, thereby affecting chemoresistance.

conclusionsIn summary, our study reveals that HOXC11 regulates mitochondrial function through the modulation of mtDNA transcription, impacting chemoresistance in colorectal cancer cells. These findings underscore the significance of understanding the molecular mechanisms underlying chemoresistance and highlight the potential therapeutic implications of targeting mitochondrial function in CRC treatment.

Indexed as

Colorectal NeoplasmsDrug Resistance, NeoplasmHomeodomain ProteinsMitochondriaCell Line, TumorCell RespirationDNA, MitochondrialGene Expression Regulation, NeoplasticHumansPrognosisTranscription, GeneticDNA, MitochondrialHomeodomain ProteinsHOXC11 protein, humanChemoresistanceChemotherapyColorectal cancerMitochondrial DNAMitochondrial respiration

Identifiers

PMID39080613
PMCPMC11290094

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.