Evidence map›Paper›PMID 39081623›Full record

ArticleInterface focus2024

Effects of mineralization on the hierarchical organization of collagen-a synchrotron X-ray scattering and polarized second harmonic generation study.

Keke Zheng, Jingxiao Zhong, Jingrui Hu, Eve Nebbiolo, Juan Sanchez-Weatherby, Tengteng Tang, William J Landis, Junning Chen, Peter Winlove, Benjamin E Sherlock and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Interface focus, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Keke ZhengBiomedical Engineering, Faculty of Environment, Science and Economy, University of Exeter, Exeter, UK.ORCID https://orcid.org/0009-0007-0470-5699
Jingxiao ZhongSchool of Aerospace, Mechanical and Mechatronic Engineering, University of Sydney, Sydney, Australia.
Jingrui HuBiomedical Engineering, Faculty of Environment, Science and Economy, University of Exeter, Exeter, UK.
Eve NebbioloBiomedical Engineering, Faculty of Environment, Science and Economy, University of Exeter, Exeter, UK.
Juan Sanchez-WeatherbyVMX-I beamline, Diamond Light Source, Didcot, UK.
Tengteng TangMaterials Science & Engineering, McMaster University, Hamilton, Ontario, Canada.
William J LandisPreventive and Restorative Dental Sciences, School of Dentistry, University of California at San Francisco, San Francisco, CA, USA.
Junning ChenBiomedical Engineering, Faculty of Environment, Science and Economy, University of Exeter, Exeter, UK.
Peter WinlovePhysics and Astronomy, Faculty of Environment, Science and Economy, University of Exeter, Exeter, UK.
Benjamin E SherlockPhysics and Astronomy, Faculty of Environment, Science and Economy, University of Exeter, Exeter, UK.
James BellSchool of Optometry and Vision Sciences, Cardiff University, Cardiff, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The process of mineralization fundamentally alters collagenous tissue biomechanics. While the structure and organization of mineral particles have been widely studied, the impact of mineralization on collagen matrix structure, particularly at the molecular scale, requires further investigation. In this study, synchrotron X-ray scattering (XRD) and polarization-resolved second harmonic generation microscopy (pSHG) were used to study normally mineralizing turkey leg tendon in tissue zones representing different stages of mineralization. XRD data demonstrated statistically significant differences in collagen D-period, intermolecular spacing, fibril and molecular dispersion and relative supramolecular twists between non-mineralizing, early mineralizing and late mineralizing zones. pSHG analysis of the same tendon zones showed the degree of collagen fibril organization was significantly greater in early and late mineralizing zones compared to non-mineralizing zones. The combination of XRD and pSHG data provide new insights into hierarchical collagen-mineral interactions, notably concerning possible cleavage of intra- or interfibrillar bonds, occlusion and reorganization of collagen by mineral with time. The complementary application of XRD and fast, label-free and non-destructive pSHG optical measurements presents a pathway for future investigations into the dynamics of molecular scale changes in collagen in the presence of increasing mineral deposition.

Indexed as

collagenmineralizationnonlinear microscopypolarization resolvedsecond harmonic generationX-ray diffraction

Identifiers

PMID39081623
PMCPMC11285761

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.