Evidence map›Paper›PMID 39083114›Full record

ArticleBiotechnology letters2024

Multiple genes deletion based on Cre-loxP marker-less gene deletion system for the strains from the genus of Pectobacterium.

Shu Che, Yuanxu Zhuo, Liping Yang, Huan Wang, Zhongli Cui, Jiaqin Fan

Abstract read
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In one paragraph

Article in Biotechnology letters, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Shu CheLaboratory of Bacteriology, Department of Plant Pathology, Nanjing Agricultural University, Nanjing, 210095, China.
Yuanxu ZhuoLaboratory of Bacteriology, Department of Plant Pathology, Nanjing Agricultural University, Nanjing, 210095, China.
Liping YangLaboratory of Bacteriology, Department of Plant Pathology, Nanjing Agricultural University, Nanjing, 210095, China.
Huan WangLaboratory of Bacteriology, Department of Plant Pathology, Nanjing Agricultural University, Nanjing, 210095, China.
Zhongli CuiKey Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences of Nanjing Agricultural University, Nanjing, 210095, China.
Jiaqin FanLaboratory of Bacteriology, Department of Plant Pathology, Nanjing Agricultural University, Nanjing, 210095, China. fanjq@njau.edu.cn.ORCID http://orcid.org/0000-0002-0547-8965

Funding

Key Laboratory in Science and Technology Development Project of Suzhou SNG2020045National Key Research and Development Program of China 2021YFC2600602National Natural Science Foundation of China 32302321
6 · The paper itself

Abstract

objectiveTo introduce the Cre-loxP system for constructing marker-less multiple-gene deletion mutants in Pectobacterium, overcoming limitations of antibiotic markers and enhancing the understanding of pathogenic mechanisms.

resultsFirstly, a plasmid named pEX18-Cre, containing a sacB sucrose suicide gene, was constructed to express Cre recombinase in Pectobacterium. Secondly, a mutant in which the loxP-Km fragment replaced the target gene was obtained through homologous recombination double-crossover with the chromosome. Finally, pEX18-Cre was introduced into the mutant to excise the DNA between the loxP sites, thereby removing the markers and achieving multiple gene deletions. By utilizing the Cre-loxP system, we successfully constructed multiple marker-less gene deletion mutants in Pectobacterium strains.

conclusionsThe Cre-loxP system efficiently creates marker-less multiple-gene deletion mutants, enhancing the study of Pectobacterium pathogenic mechanisms by overcoming antibiotic marker limitations.

Indexed as

Gene DeletionIntegrasesPectobacteriumGenetic MarkersHomologous RecombinationPlasmidsCre recombinaseGenetic MarkersIntegrasesAntibiotic markerCre-loxP multiple genes deletionPectobacterium

Identifiers

PMID39083114

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.