Evidence map›Paper›PMID 39091806›Full record

ArticlebioRxiv : the preprint server for biology2024

Sirt5 regulates chondrocyte metabolism and osteoarthritis development through protein lysine malonylation.

Huanhuan Liu, Anupama Binoy, Siqi Ren, Thomas C Martino, Anna E Miller, Craig R G Willis, Shivakumar R Veerabhadraiah, Abhijit Sukul, Joanna Bons, Jacob P Rose and 3 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Huanhuan LiuDepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine (HCOM), Ohio University, Athens, OH, 45701, USA.
Anupama BinoyDepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine (HCOM), Ohio University, Athens, OH, 45701, USA.
Siqi RenDepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine (HCOM), Ohio University, Athens, OH, 45701, USA.
Thomas C MartinoDepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine (HCOM), Ohio University, Athens, OH, 45701, USA.
Anna E MillerDepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine (HCOM), Ohio University, Athens, OH, 45701, USA.
Craig R G WillisSchool of Chemistry and Biosciences, Faculty of Life Sciences, University of Bradford, Bradford, BD7 1DP, UK.
Shivakumar R VeerabhadraiahDepartment of Orthopaedics, University of Utah, Salt Lake City, Utah, 84108 USA.
Abhijit SukulDepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine (HCOM), Ohio University, Athens, OH, 45701, USA.
Joanna BonsBuck Institute for Research on Aging, 8001 Redwood Boulevard, Novato, CA 94945, USA.
Jacob P RoseBuck Institute for Research on Aging, 8001 Redwood Boulevard, Novato, CA 94945, USA.
Birgit SchillingBuck Institute for Research on Aging, 8001 Redwood Boulevard, Novato, CA 94945, USA.ORCID 0000-0001-9907-2749
Michael J JurynecDepartment of Orthopaedics, University of Utah, Salt Lake City, Utah, 84108 USA.
Shouan ZhuDepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine (HCOM), Ohio University, Athens, OH, 45701, USA.ORCID 0000-0002-4973-1504

Funding

Neurobiology Core CP30AR079206 · NIAMS · RUSH UNIVERSITY MEDICAL CENTER · PI Anne-Marie Malfait · 2021 to 2026
$4.7M
Growth hormone regulating chondrocyte metabolism for osteoarthritis developmentR01AR081804 · NIAMS · OHIO UNIVERSITY ATHENS · PI Shouan Zhu · 2023 to 2026
$2.0M
Contribution of the NOD/RIPK2 signaling pathway to osteoarthritis susceptibilityR01AR082973 · NIAMS · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · PI MICHAEL J JURYNEC · 2024 to 2026
$1.7M
Orbitrap Fusion Lumos ETD with FAIMS ProS10OD028654 · OD · BUCK INSTITUTE FOR RESEARCH ON AGING · PI SCHILLING, BIRGIT · 2020 to 2020
$1.1M
Obesity promoting protein malonylation and chondrocyte metabolic dysfunction in osteoarthritis developmentR15AR080813 · NIAMS · OHIO UNIVERSITY ATHENS · PI ZHU, SHOUAN · 2022 to 2022
$453k
NIAMS NIH HHS P30 AR079206NIAMS NIH HHS R01 AR081804NIAMS NIH HHS R01 AR082973NIAMS NIH HHS R15 AR080813NIH HHS S10 OD028654
6 · The paper itself

Abstract

Objectives: Chondrocyte metabolic dysfunction plays an important role in osteoarthritis (OA) development during aging and obesity. Protein post-translational modifications (PTMs) have recently emerged as an important regulator of cellular metabolism. We aim to study one type of PTM, lysine malonylation (MaK) and its regulator Sirt5 in OA development. Methods: Human and mouse cartilage tissues were used to measure SIRT5 and MaK levels. Both systemic and cartilage-specific conditional knockout mouse models were subject to high-fat diet (HFD) treatment to induce obesity and OA. Proteomics analysis was performed in Results: We found that SIRT5 decreases while MAK increases in the cartilage during aging. A combination of Sirt5 deficiency and obesity exacerbates joint degeneration in a sex dependent manner in mice. We further delineate the malonylome in chondrocytes, pinpointing MaK's predominant impact on various metabolic pathways such as carbon metabolism and glycolysis. Lastly, we identified a rare coding mutation in Conclusions: We found that Sirt5 mediated MaK is an important regulator of chondrocyte cellular metabolism and dysregulation of Sirt5-MaK could be an important mechanism underlying aging and obesity associated OA development.

Identifiers

PMID39091806
PMCPMC11291161

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.