ArticlebioRxiv : the preprint server for biology2025
DNA O-MAP uncovers the molecular neighborhoods associated with specific genomic loci.
Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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15 authors.
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Abstract
The accuracy of crucial nuclear processes such as transcription, replication, and repair, depends on the local composition of chromatin and the regulatory proteins that reside there. Understanding these DNA-protein interactions at the level of specific genomic loci has remained challenging due to technical limitations. Here, we introduce a method termed "DNA O-MAP", which uses programmable peroxidase-conjugated oligonucleotide probes to biotinylate nearby proteins. We show that DNA O-MAP can be coupled with sample multiplexed quantitative proteomics, targeted chemical perturbations, and next-generation sequencing to quantify DNA-protein and DNA-DNA interactions at specific genomic loci. Furthermore, we establish that DNA O-MAP \ is applicable to both repetitive and unique genomic loci of varying sizes (kilobases to megabases), and that DNA O-MAP can measure proximal molecular effectors in a homolog-specific manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.