Evidence map›Paper›PMID 39091855›Full record

ArticlebioRxiv : the preprint server for biology2024

Proteomic Profiling Reveals Age-Related Changes in Transporter Proteins in the Human Blood-Brain Barrier.

Xujia Zhou, Mina Azimi, Niklas Handin, Andrew Riselli, Bianca Vora, Eden Chun, Sook Wah Yee, Per Artursson, Kathleen M Giacomini

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Xujia ZhouDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California, United States.ORCID 0000-0003-2491-0933
Mina AzimiDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California, United States.ORCID 0000-0001-9107-3560
Niklas HandinDepartment of Pharmacy, Uppsala University, Uppsala, Sweden.ORCID 0000-0003-1838-0681
Andrew RiselliDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California, United States.ORCID 0000-0002-0714-5726
Bianca VoraDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California, United States.ORCID 0000-0002-0501-1804
Eden ChunDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California, United States.
Sook Wah YeeDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California, United States.ORCID 0000-0001-8121-8746
Per ArturssonDepartment of Pharmacy, Uppsala University, Uppsala, Sweden.ORCID 0000-0002-3708-7395
Kathleen M GiacominiDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California, United States.ORCID 0000-0001-8041-5430

Funding

CLINICAL, PHARMACOLOGY, DRUG ACTION &PHARMACOKINETICST32GM007546 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Radojka Savic · 1985 to 2026
$8.9M
UCSF-Stanford Center of Excellence in Regulatory Science and InnovationU01FD004979 · FDA · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ALTMAN, RUSS BIAGIO, GIACOMINI, KATHLEEN M · 2014 to 2016
$5.4M
Transporter Elucidation Center at the University of California, San FranciscoUC2HD113474 · NICHD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KATHLEEN M GIACOMINI, Robert M Stroud · 2023 to 2026
$3.9M
FDA HHS U01 FD004979FDA HHS U01 FD005978NICHD NIH HHS UC2 HD113474NIGMS NIH HHS T32 GM007546
6 · The paper itself

Abstract

The Blood-Brain Barrier (BBB) is a crucial, selective barrier that regulates the entry of molecules including nutrients, environmental toxins, and therapeutic medications into the brain. This function relies heavily on brain endothelial cell proteins, particularly transporters and tight junction proteins. The BBB continues to develop postnatally, adapting its selective barrier function across different developmental phases, and alters with aging and disease. Here we present a global proteomics analysis focused on the ontogeny and aging of proteins in human brain microvessels (BMVs), predominantly composed of brain endothelial cells. Our proteomic profiling quantified 6,223 proteins and revealed possible age-related alteration in BBB permeability due to basement membrane component changes through the early developmental stage and age-dependent changes in transporter expression. Notable changes in expression levels were observed with development and age in nutrient transporters and transporters that play critical roles in drug disposition. This research 1) provides important information on the mechanisms that drive changes in the metabolic content of the brain with age and 2) enables the creation of physiologically based pharmacokinetic models for CNS drug distribution across different life stages.

Identifiers

PMID39091855
PMCPMC11291171

What Socratic holds

Textmetadata
LicenceCC BY-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.