Evidence map›Paper›PMID 39092897›Full record

ArticleExperimental physiology2024

Circulating extracellular microvesicles associated with electronic cigarette use increase endothelial cell inflammation and reduce nitric oxide production.

Nicholas G Evanoff, Donald R Dengel, Kelly A Stockelman, Hannah Fandl, Noah M DeSouza, Jared J Greiner, Sheena R Dufresne, Michael Kotlyar, Vinicius P Garcia

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental physiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Nicholas G EvanoffSchool of Kinesiology, University of Minnesota, Minneapolis, Minnesota, USA.ORCID https://orcid.org/0000-0001-5616-5857
Donald R DengelSchool of Kinesiology, University of Minnesota, Minneapolis, Minnesota, USA.
Kelly A StockelmanDepartment of Integrative Physiology, University of Colorado, Boulder, Colorado, USA.
Hannah FandlDepartment of Integrative Physiology, University of Colorado, Boulder, Colorado, USA.
Noah M DeSouzaDepartment of Integrative Physiology, University of Colorado, Boulder, Colorado, USA.
Jared J GreinerDepartment of Integrative Physiology, University of Colorado, Boulder, Colorado, USA.
Sheena R DufresneDepartment of Experimental and Clinical Pharmacology, University of Minnesota, Minneapolis, Minnesota, USA.
Michael KotlyarDepartment of Experimental and Clinical Pharmacology, University of Minnesota, Minneapolis, Minnesota, USA.
Vinicius P GarciaDepartment of Integrative Physiology, University of Colorado, Boulder, Colorado, USA.

Funding

UMN | College of Education and Human Development, University of Minnesota (CEHD, UM)
6 · The paper itself

Abstract

The purpose of this study was to determine the effect of circulating microvesicles isolated from chronic electronic (e-)cigarette users on cultured human umbilical vein endothelial cell (HUVEC) expression of nuclear factor-κB (NF-κB), cellular cytokine release, phosphorylation of endothelial nitric oxide synthase (eNOS) and NO production. The HUVECs were treated with microvesicles isolated via flow cytometry from nine non-tobacco users (five male and four female; 22 ± 2 years of age) and 10 e-cigarette users (six male and four female; 22 ± 2 years of age). Microvesicles from e-cigarette users induced significantly greater release of interleukin-6 (183.4 ± 23.6 vs. 150.6 ± 15.4 pg/mL; P = 0.002) and interleukin-8 (160.0 ± 31.6 vs. 129.4 ± 11.2 pg/mL; P = 0.01), in addition to expression of p-NF-κB p65 (Ser536) (18.8 ± 3.4 vs. 15.6 ± 1.5 a.u.; P = 0.02) from HUVECs compared with microvesicles from non-tobacco users. Nuclear factor-κB p65 was not significantly different between microvesicles from the non-tobacco users and from the e-cigarette users (87.6 ± 8.7 vs. 90.4 ± 24.6 a.u.; P = 0.701). Neither total eNOS (71.4 ± 21.8 vs. 80.4 ± 24.5 a.u.; P = 0.413) nor p-eNOS (Thr495) (229.2 ± 26.5 vs. 222.1 ± 22.7 a.u.; P = 0.542) was significantly different between microvesicle-treated HUVECs from non-tobacco users and e-cigarette users. However, p-eNOS (Ser1177) (28.9 ± 6.2 vs. 45.8 ± 9.0 a.u.; P < 0.001) expression was significantly lower from e-cigarette users compared with non-tobacco users. Nitric oxide production was significantly lower (8.2 ± 0.6 vs. 9.7 ± 0.9 μmol/L; P = 0.001) in HUVECs treated with microvesicles from e-cigarette users compared with microvesicles from non-tobacco users. This study demonstrated increased NF-κB activation and inflammatory cytokine production, in addition to diminished eNOS activity and NO production resulting from e-cigarette use. HIGHLIGHTS: What is the central question of this study? Circulating microvesicles contribute to cardiovascular health and disease via their effects on the vascular endothelium. The impact of electronic (e-)cigarette use on circulating microvesicle phenotype is not well understood. What is the main finding and its importance? Circulating microvesicles from e-cigarette users increase endothelial cell inflammation and impair endothelial nitric oxide production. Endothelial inflammation and diminished nitric oxide bioavailability are central factors underlying endothelial dysfunction and, in turn, cardiovascular disease risk. Deleterious changes in the functional phenotype of circulating microvesicles might contribute to the reported adverse effects of e-cigarette use on cardiovascular health.

Indexed as

Cell-Derived MicroparticlesElectronic Nicotine Delivery SystemsHuman Umbilical Vein Endothelial CellsInflammationNF-kappa BNitric OxideNitric Oxide Synthase Type IIIAdultCells, CulturedCytokinesFemaleHumansInterleukin-6Interleukin-8MalePhosphorylationCytokinesInterleukin-6Interleukin-8NF-kappa BNitric OxideNitric Oxide Synthase Type IIINOS3 protein, humanelectronic cigaretteendothelial cellendothelial nitric oxide synthaseinflammationmicrovesicle

Identifiers

PMID39092897
PMCPMC11363099

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.