Evidence map›Paper›PMID 39095365›Full record

ArticleNature communications2024

Transcriptional regulation of the postnatal cardiac conduction system heterogeneity.

Yena Oh, Rimshah Abid, Saif Dababneh, Marwan Bakr, Termeh Aslani, David P Cook, Barbara C Vanderhyden, Jin G Park, Nikhil V Munshi, Chi-Chung Hui and 1 more

Abstract read
In one paragraph

Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Article
  5. International journal of molecular sciences · 2026
    Article
  6. Isolation and Culture of Neonatal Mouse Atrial and Ventricular Myocytes.Methods in molecular biology (Clifton, N.J.) · 2026
    Article
  7. Review
  8. Article
  9. Review
  10. Review
  11. Review
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yena OhUniversity of Ottawa Heart Institute, Ottawa, ON, Canada.ORCID 0000-0003-0871-6989
Rimshah AbidUniversity of Ottawa Heart Institute, Ottawa, ON, Canada.ORCID 0000-0002-2609-0767
Saif DababnehUniversity of Ottawa Heart Institute, Ottawa, ON, Canada.ORCID 0000-0003-1593-0756
Marwan BakrUniversity of Ottawa Heart Institute, Ottawa, ON, Canada.ORCID 0009-0004-2902-2024
Termeh AslaniUniversity of Ottawa Heart Institute, Ottawa, ON, Canada.ORCID 0000-0003-3181-7550
David P CookDepartment of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
Barbara C VanderhydenDepartment of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.ORCID 0000-0002-7644-7189
Jin G ParkVirginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ, USA.ORCID 0000-0001-6481-9590
Nikhil V MunshiDepartment of Internal Medicine, Division of Cardiology, UT Southwestern Medical Center, Dallas, TX, USA.ORCID 0000-0002-8397-100X
Chi-Chung HuiProgram in Developmental & Stem Cell Biology, The Hospital for Sick Children, Toronto, ON, Canada.ORCID 0000-0001-7047-6694
Kyoung-Han KimUniversity of Ottawa Heart Institute, Ottawa, ON, Canada. hankim@uottawa.ca.ORCID 0000-0002-0433-656X

Funding

Exploring Mechanisms of Cardiac Pacemaker Cell Fate DeterminationR01HL151650 · NHLBI · UT SOUTHWESTERN MEDICAL CENTER · PI MUNSHI, NIKHIL VILAS · 2021 to 2025
$2.1M
Gouvernement du Canada | Canadian Institutes of Health Research (Instituts de Recherche en Santé du Canada) CGS-DGouvernement du Canada | Canadian Institutes of Health Research (Instituts de Recherche en Santé du Canada) CGS-MGouvernement du Canada | Canadian Institutes of Health Research (Instituts de Recherche en Santé du Canada) PJT 173281Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (Conseil de Recherches en Sciences Naturelles et en Génie du Canada) RGPIN-2018-06870Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (Conseil de Recherches en Sciences Naturelles et en Génie du Canada) USRAHeart and Stroke Foundation of Canada (Heart and Stroke Foundation) NNIANHLBI NIH HHS R01 HL151650
6 · The paper itself

Abstract

The cardiac conduction system (CCS) is a network of specialized cardiomyocytes that coordinates electrical impulse generation and propagation for synchronized heart contractions. Although the components of the CCS, including the sinoatrial node, atrioventricular node, His bundle, bundle branches, and Purkinje fibers, were anatomically discovered more than 100 years ago, their molecular constituents and regulatory mechanisms remain incompletely understood. Here, we demonstrate the transcriptomic landscape of the postnatal mouse CCS at a single-cell resolution with spatial information. Integration of single-cell and spatial transcriptomics uncover region-specific markers and zonation patterns of expression. Network inference shows heterogeneous gene regulatory networks across the CCS. Notably, region-specific gene regulation is recapitulated in vitro using neonatal mouse atrial and ventricular myocytes overexpressing CCS-specific transcription factors, Tbx3 and/or Irx3. This finding is supported by ATAC-seq of different CCS regions, Tbx3 ChIP-seq, and Irx motifs. Overall, this study provides comprehensive molecular profiles of the postnatal CCS and elucidates gene regulatory mechanisms contributing to its heterogeneity.

Indexed as

Heart Conduction SystemHomeodomain ProteinsMyocytes, CardiacT-Box Domain ProteinsAnimalsAnimals, NewbornAtrioventricular NodeBundle of HisGene Expression RegulationGene Regulatory NetworksMicePurkinje FibersSingle-Cell AnalysisSinoatrial NodeTranscription FactorsTranscriptomeHomeodomain ProteinsT-Box Domain ProteinsTbx3 protein, mouseTranscription Factors

Identifiers

PMID39095365
PMCPMC11297185

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.