Evidence map›Paper›PMID 39095385›Full record

ArticleNature communications2024

Single-cell RNA sequencing reveals placental response under environmental stress.

Eric Van Buren, David Azzara, Javier Rangel-Moreno, Maria de la Luz Garcia-Hernandez, Shawn P Murphy, Ethan D Cohen, Ethan Lewis, Xihong Lin, Hae-Ryung Park

Abstract read
In one paragraph

Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Subtype specific immune-metabolic reprogramming in preeclampsia revealed by multiomics and serum biomarkers.Hypertension research : official journal of the Japanese Society of Hypertension · 2026
    Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Review
  15. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Eric Van BurenDepartment of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, USA.
David AzzaraDepartment of Environmental Medicine, School of Medicine and Dentistry, University of Rochester, Rochester, NY, USA.ORCID 0009-0001-1642-9798
Javier Rangel-MorenoDivision of Allergy, Immunology and Rheumatology, Department of Medicine, University of Rochester, Rochester, NY, USA.ORCID 0000-0002-9738-1182
Maria de la Luz Garcia-HernandezDivision of Allergy, Immunology and Rheumatology, Department of Medicine, University of Rochester, Rochester, NY, USA.ORCID 0000-0001-9268-2464
Shawn P MurphyDepartment of Obstetrics and Gynecology, School of Medicine and Dentistry, University of Rochester, Rochester, NY, USA.
Ethan D CohenDepartment of Pediatrics, School of Medicine and Dentistry, University of Rochester, Rochester, NY, USA.ORCID 0000-0003-4914-880X
Ethan LewisDepartment of Environmental Medicine, School of Medicine and Dentistry, University of Rochester, Rochester, NY, USA.ORCID 0009-0003-2135-1424
Xihong LinDepartment of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, USA.ORCID 0000-0001-7067-7752
Hae-Ryung ParkDepartment of Environmental Medicine, School of Medicine and Dentistry, University of Rochester, Rochester, NY, USA. hae-ryung_park@urmc.rochester.edu.ORCID 0000-0002-7531-8844

Funding

Translational Research Support CoreP30ES000002 · NIEHS · HARVARD UNIVERSITY (SCH OF PUBLIC HLTH) · PI Brent Andrew Coull · 1985 to 2026
$44.6M
VISUAL INDICES OF NEUROTOXICITYP30ES001247 · NIEHS · UNIVERSITY OF ROCHESTER · PI Katrina S Korfmacher · 1985 to 2026
$42.8M
Training in Environment ToxicologyT32ES007026 · NIEHS · UNIVERSITY OF ROCHESTER · PI Alison Elder, Marissa Sobolewski Terry · 1985 to 2026
$20.2M
GRADUATE TRAINING IN BIOSTATISTICST32ES007142 · NIEHS · HARVARD UNIVERSITY (SCH OF PUBLIC HLTH) · PI Brent Andrew Coull · 1985 to 2026
$11.9M
ROLE OF INDUCIBLE BRONCHUS ASSOCIATED LYMPHOID TISSUE IN LATENT TUBERCULOSISR01AI111914 · NIAID · WASHINGTON UNIVERSITY · PI KAUSHAL, DEEPAK, KHADER, SHABAANA A. · 2015 to 2025
$11.1M
Spatial patterns of metals and metal mixtures in drinking waterP42ES030990 · NIEHS · HARVARD UNIVERSITY D/B/A HARVARD SCHOOL OF PUBLIC HEALTH · PI LU, QUAN · 2020 to 2024
$8.3M
Predictive Modeling of the Functional and Phenotypic Impacts of Genetic VariantsU01HG012064 · NHGRI · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI Manuel Garber, XIHONG LIN · 2021 to 2026
$4.0M
Statistical Methods for Integrative Analysis of Large-Scale Whole Genome Sequencing Studies and Biobanks of Common DiseasesR01HL163560 · NHLBI · HARVARD UNIVERSITY D/B/A HARVARD SCHOOL OF PUBLIC HEALTH · PI XIHONG LIN · 2022 to 2026
$2.6M
MicroRNAs, ER Stress and Arsenic NeurotoxicityR00ES029548 · NIEHS · UNIVERSITY OF ROCHESTER · PI PARK, HAE-RYUNG · 2021 to 2023
$747k
MicroRNAs, ER stress and arsenic neurotoxicityK99ES029548 · NIEHS · HARVARD SCHOOL OF PUBLIC HEALTH · PI PARK, HAE-RYUNG · 2019 to 2020
$203k
NHGRI NIH HHS U01 HG012064NHLBI NIH HHS R01 HL163560NIAID NIH HHS R01 AI111914NIEHS NIH HHS K99 ES029548NIEHS NIH HHS P30 ES000002NIEHS NIH HHS P30 ES001247NIEHS NIH HHS P42 ES030990NIEHS NIH HHS R00 ES029548NIEHS NIH HHS T32 ES007026NIEHS NIH HHS T32 ES007142U.S. Department of Health & Human Services | NIH | National Institute of Environmental Health Sciences (NIEHS) P30ES001247U.S. Department of Health & Human Services | NIH | National Institute of Environmental Health Sciences (NIEHS) R00ES029548
6 · The paper itself

Abstract

The placenta is crucial for fetal development, yet the impact of environmental stressors such as arsenic exposure remains poorly understood. We apply single-cell RNA sequencing to analyze the response of the mouse placenta to arsenic, revealing cell-type-specific gene expression, function, and pathological changes. Notably, the Prap1 gene, which encodes proline-rich acidic protein 1 (PRAP1), is significantly upregulated in 26 placental cell types including various trophoblast cells. Our study shows a female-biased increase in PRAP1 in response to arsenic and localizes it in the placenta. In vitro and ex vivo experiments confirm PRAP1 upregulation following arsenic treatment and demonstrate that recombinant PRAP1 protein reduces arsenic-induced cytotoxicity and downregulates cell cycle pathways in human trophoblast cells. Moreover, PRAP1 knockdown differentially affects cell cycle processes, proliferation, and cell death depending on the presence of arsenic. Our findings provide insights into the placental response to environmental stress, offering potential preventative and therapeutic approaches for environment-related adverse outcomes in mothers and children.

Indexed as

ArsenicPlacentaSingle-Cell AnalysisTrophoblastsAnimalsCell CycleCell ProliferationFemaleHumansMiceMice, Inbred C57BLPregnancySequence Analysis, RNAStress, PhysiologicalUp-RegulationArsenic

Identifiers

PMID39095385
PMCPMC11297347

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.