Evidence map›Paper›PMID 39097092›Full record

ArticleJournal of advanced research2025

ADAM8 deficiency in macrophages promotes cardiac repair after myocardial infarction via ANXA2-mTOR-autophagy pathway.

Zhenjun Ji, Jiaqi Guo, Rui Zhang, Wenjie Zuo, Yang Xu, Yangyang Qu, Zaixiao Tao, Xinxin Li, Yongjun Li, Yuyu Yao and 1 more

Abstract read
In one paragraph

Article in Journal of advanced research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Trial
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  9. Article
  10. mTOR Signaling in Macrophages: All Depends on the Context.International journal of molecular sciences · 2025
    Review
  11. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Zhenjun JiDepartment of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, Jiangsu, China.
Jiaqi GuoDepartment of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, Jiangsu, China.
Rui ZhangDepartment of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, Jiangsu, China.
Wenjie ZuoDepartment of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, 330008, Jiangxi, China.
Yang XuDepartment of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, Jiangsu, China.
Yangyang QuDepartment of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, Jiangsu, China.
Zaixiao TaoDepartment of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, Jiangsu, China.
Xinxin LiDepartment of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, Jiangsu, China.
Yongjun LiDepartment of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, Jiangsu, China.
Yuyu YaoDepartment of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, Jiangsu, China.
Genshan MaDepartment of Cardiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 210009, Jiangsu, China. Electronic address: magenshan@seu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionA disintegrin and metalloproteinase 8 (ADAM8), a crucial regulator in macrophages, is closely associated with cardiovascular disease progression.

objectivesThis study aimed to explore how ADAM8 regulates macrophage function to inhibit cardiac repair after myocardial infarction (MI).

methodsMacrophage-specific ADAM8 knockout mice (ADAM8

resultsADAM8 was highly expressed in the plasma of patients with acute myocardial infarction (AMI) and in cardiac macrophages derived from AMI mice. ADAM8 KO mice exhibited enhanced angiogenesis, suppressed inflammation, reduced cardiac fibrosis, and improved cardiac function during AMI, which were reversed by overexpressing macrophage-specific ADAM8 and intervention with the clinical anti-angiogenic biologic bevacizumab. Bone marrow transplantation experiments produced ADAM8 KO phenotypes. RNA sequencing showed that autophagy was activated in bone marrow-derived macrophages (BMDMs) with ADAM8 KO, which was confirmed via p-mTOR Ser2448/mTOR, p62, and LC3II/I detection. Autophagy inactivation suppressed angiogenic factor release and promoted inflammation in BMDMs with ADAM8 KO. Mechanistically, ADAM8 could bind to ANXA2 and promote phosphorylation of the ANXA2 Ser26 site. ADAM8 KO impeded ANXA2 phosphorylation, inhibited mTOR Ser2448 site phosphorylation, and activated autophagy, which were demonstrated using the activation or inactivation of ANXA2 phosphorylation.

conclusionsADAM8 was increased in cardiac macrophages after AMI. The ADAM8-ANXA2-mTOR-autophagy axis in macrophages is responsible for regulating angiogenesis and inflammation following MI. Thus, ADAM8 may be a new target in MI treatment.

Indexed as

ADAM ProteinsAnnexin A2AutophagyMacrophagesMembrane ProteinsMyocardial InfarctionTOR Serine-Threonine KinasesAnimalsAntigens, CDDisease Models, AnimalHumansMaleMiceMice, Inbred C57BLMice, KnockoutSignal TransductionADAM8 protein, humanAdam8 protein, mouseADAM ProteinsAnnexin A2Antigens, CDMembrane ProteinsmTOR protein, mouseTOR Serine-Threonine KinasesADAM8AngiogenesisInflammationMacrophageMyocardial infarction

Identifiers

PMID39097092
PMCPMC12225930

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.