Evidence map›Paper›PMID 39102162›Full record

ArticleAnalytical sciences : the international journal of the Japan Society for Analytical Chemistry2024

Design of deep-red emissive forced intercalation-induced light-up peptide as an indicator for the HIV-1 TAR RNA-ligand assay: integration of benzo[c,d]indole-quinoline (BIQ) cyanine dye into Tat peptide.

Akunna Francess Ujuagu, Yusuke Sato, En Ting Tabitha Lee, Seiichi Nishizawa

Abstract read
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In one paragraph

Article in Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Design of triplex-forming peptide nucleic acid-based fluorescent probes for forced intercalation sensing of double-stranded RNA structures.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Akunna Francess UjuaguDepartment of Chemistry, Graduate School of Science, Tohoku University, Aoba-Ku, Sendai, 980-8578, Japan.
Yusuke SatoDepartment of Chemistry, Graduate School of Science, Tohoku University, Aoba-Ku, Sendai, 980-8578, Japan. yusuke.sato.a7@tohoku.ac.jp.ORCID http://orcid.org/0000-0002-5824-1654
En Ting Tabitha LeeDepartment of Chemistry, Graduate School of Science, Tohoku University, Aoba-Ku, Sendai, 980-8578, Japan.
Seiichi NishizawaDepartment of Chemistry, Graduate School of Science, Tohoku University, Aoba-Ku, Sendai, 980-8578, Japan. seiichi.nishizawa.c8@tohoku.ac.jp.ORCID https://orcid.org/0000-0001-6303-2999

Funding

Japan Society for the Promotion of Science 22H02099Japan Society for the Promotion of Science 23H00297
6 · The paper itself

Abstract

We report on a deep-red emissive fluorogenic peptide probe for human immunodeficiency virus-1 (HIV-1) trans-activation responsive (TAR) RNA as an indicator for fluorescence indicator displacement (FID) assay. The probe design is based on the concept of the forced intercalation of thiazole orange (TO) dyes (FIT) on the peptide backbone, as recently proposed by our group, where the Q (glutamic acid) residue in the Tat peptide (RKKRR-Q-RRR) is replaced with TO as if it were an amino acid surrogate. Here, instead of green emissive TO, we utilized a deep-red emissive benzo[c,d]indole-quinoline (BIQ) cyanine dye developed previously by our group for imaging of nucleolar RNA in living cells. The developed 9-mer FIT peptide (RKKRR-BIQ-RRR; named BIQ-FiLuP) exhibits a significant off-on signaling ability for TAR RNA (λ

Indexed as

Fluorescent DyesHIV-1QuinolinesRNA, Viraltat Gene Products, Human Immunodeficiency VirusBenzothiazolesCarbocyaninesHIV Long Terminal RepeatHumansIntercalating AgentsLigandsPeptidesBenzothiazolesCarbocyaninesFluorescent DyesIntercalating AgentsLigandsPeptidesQuinolinesRNA, Viraltat Gene Products, Human Immunodeficiency VirusCyanine dyeDeep-red emissionDrug discoveryFID assayFluorescent probeRNA

Identifiers

PMID39102162

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.