Evidence map›Paper›PMID 39103560›Full record

ArticleHuman cell2024

Development of 3D-iNET ORION: a novel, pre-clinical, three-dimensional in vitro cell model for modeling human metastatic neuroendocrine tumor of the pancreas.

Jan Strnadel, Mark A Valasek, Grace Y Lin, Huahui Lin, Ann M Ponsford Tipps, Sang Myung Woo, Ken Fujimura, Huawei Wang, Sunkyu Choi, Jack Bui and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Human cell, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Jan Strnadel *Jessenius Faculty of Medicine in Martin, Biomedical Centre Martin, Comenius University in Bratislava, 036 01, Martin, Slovakia. jan.strnadel@uniba.sk.ORCID http://orcid.org/0000-0002-7233-6537
Mark A Valasek *Department of Pathology, University of California, La Jolla, San Diego, CA, USA.
Grace Y LinDepartment of Pathology, University of California, La Jolla, San Diego, CA, USA.
Huahui LinDepartment of Pathology, University of California, La Jolla, San Diego, CA, USA.
Ann M Ponsford TippsDepartment of Pathology, University of California, La Jolla, San Diego, CA, USA.
Sang Myung WooDepartment of Pathology, University of California, La Jolla, San Diego, CA, USA.
Ken FujimuraDepartment of Pathology, University of California, La Jolla, San Diego, CA, USA.
Huawei WangDepartment of Pathology, University of California, La Jolla, San Diego, CA, USA.
Sunkyu ChoiDepartment of Pathology, University of California, La Jolla, San Diego, CA, USA.
Jack BuiDepartment of Pathology, University of California, La Jolla, San Diego, CA, USA.
Christopher HermosilloInstitute for Genomic Medicine, University of California, La Jolla, San Diego, CA, USA.
Kristen JepsenInstitute for Genomic Medicine, University of California, La Jolla, San Diego, CA, USA.
Michael R NavarroNeuroregeneration Laboratory, Department of Anesthesiology, University of California San Diego, La Jolla, San Diego, CA, USA.
Jonathan A KelberDepartment of Pathology, University of California, La Jolla, San Diego, CA, USA.
Richard L KlemkeDepartment of Pathology, University of California, La Jolla, San Diego, CA, USA.
Michael BouvetMoores Cancer Center, University of California, La Jolla, San Diego, CA, USA.

Funding

SPATIOTEMPORAL MECHANISMS OF EIF5A1/2-MEDIATED METASTASIS IN TRIPLE-NEGATIVE BREAST CANCERSC1GM121182 · NIGMS · CALIFORNIA STATE UNIVERSITY NORTHRIDGE · PI KELBER, JONATHAN · 2017 to 2024
$3.2M
Molecular Imaging Probe(s) for Optical Surgical Navigation of Pancreatic CancerR01CA256973 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Surinder K. Batra, Michael Bouvet · 2022 to 2026
$2.2M
Agentúra na Podporu Výskumu a Vývoja APVV-21-0372European Regional Development Fund ITMS: 313011AFG5NCI NIH HHS R01 CA256973NIGMS NIH HHS SC1 GM121182NIH (US) NIH SC1GM121182
6 · The paper itself

Abstract

Neuroendocrine tumors (NETs) of the pancreas are rare neoplasms that present complex challenges to diagnosis and treatment due to their indolent course. The incidence of pancreatic neuroendocrine tumors has increased significantly over the past two decades. A limited number of pancreatic neuroendocrine cell lines are currently available for the research. Here, we present 3D-iNET ORION, a novel 3-dimensional (spheroid) cell line, isolated from human pancreatic neuroendocrine tumor liver metastasis. Three-dimensionally grown (3D) cancer cell lines have gained interest over the past years as 3D cancer cell lines better recapitulate the in vivo structure of tumors, and are more suitable for in vitro and in vivo experiments. 3D-iNET ORION cancer cell line showed high potential to form tumorspheres when embedded in Matrigel matrix and expresses synaptophysin and EpCAM. Electron microscopy analysis of cancer cell line proved the presence of dense neurosecretory granules. When xenografted into athymic mice, 3D-iNET ORION cells produce slow-growing tumors, positive for chromogranin and synaptophysin. Human Core Exome Panel Analysis has shown that 3DiNET ORION cell line retains the genetic aberration profile detected in the original tumor. In conclusion, our newly developed neuroendocrine cancer cell line can be considered as a new research tool for in vitro and in vivo experiments.

Indexed as

Mice, NudeNeuroendocrine TumorsPancreatic NeoplasmsAnimalsCell Culture TechniquesCell Culture Techniques, Three DimensionalCell Line, TumorHumansLiver NeoplasmsMiceModels, BiologicalSpheroids, Cellular3D cancer cell linesNeuroendocrine tumorPancreas

Identifiers

PMID39103560
PMCPMC11341600

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.