Evidence map›Paper›PMID 39108322›Full record

ArticleHemaSphere2024

A cellular reporter system to evaluate endogenous fetal hemoglobin induction and screen for therapeutic compounds.

Thijs C J Verheul, Nynke Gillemans, Kerstin Putzker, Rezin Majied, Tingyue Li, Memnia Vasiliou, Bert Eussen, Annelies de Klein, Wilfred F J van IJcken, Emile van den Akker and 6 more

Abstract read
In one paragraph

Article in HemaSphere, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Thijs C J VerheulDepartment of Cell Biology Erasmus MC Rotterdam The Netherlands.
Nynke GillemansDepartment of Cell Biology Erasmus MC Rotterdam The Netherlands.
Kerstin PutzkerEMBL Chemical Biology Core facility Heidelberg Germany.
Rezin MajiedDepartment of Cell Biology Erasmus MC Rotterdam The Netherlands.
Tingyue LiDepartment of Cell Biology Erasmus MC Rotterdam The Netherlands.
Memnia VasiliouDepartment of Cell Biology Erasmus MC Rotterdam The Netherlands.
Bert EussenDepartment of Clinical Genetics Erasmus MC Rotterdam The Netherlands.
Annelies de KleinDepartment of Clinical Genetics Erasmus MC Rotterdam The Netherlands.
Wilfred F J van IJckenDepartment of Cell Biology Erasmus MC Rotterdam The Netherlands.
Emile van den AkkerSanquin Research Amsterdam The Netherlands.
Marieke von LindernSanquin Research Amsterdam The Netherlands.
Joe LewisEMBL Chemical Biology Core facility Heidelberg Germany.
Ulrike UhrigEMBL Chemical Biology Core facility Heidelberg Germany.
Yukio NakamuraRIKEN BioResource Center Tsukuba Ibaraki Japan.
Thamar van DijkDepartment of Cell Biology Erasmus MC Rotterdam The Netherlands.
Sjaak PhilipsenDepartment of Cell Biology Erasmus MC Rotterdam The Netherlands.ORCID 0000-0002-3690-1201

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reactivation of fetal hemoglobin expression alleviates the symptoms associated with β-globinopathies, severe hereditary diseases with significant global health implications due to their high morbidity and mortality rates. The symptoms emerge following the postnatal transition from fetal-to-adult hemoglobin expression. Extensive research has focused on inducing the expression of the fetal γ-globin subunit to reverse this switch and ameliorate these symptoms. Despite decades of research, only one compound, hydroxyurea, found its way to the clinic as an inducer of fetal hemoglobin. Unfortunately, its efficacy varies among patients, highlighting the need for more effective treatments. Erythroid cell lines have been instrumental in the pursuit of both pharmacological and genetic ways to reverse the postnatal hemoglobin switch. Here, we describe the first endogenously tagged fetal hemoglobin reporter cell line based on the adult erythroid progenitor cell line HUDEP2. Utilizing CRISPR-Cas9-mediated knock-in, a bioluminescent tag was integrated at the

Identifiers

PMID39108322
PMCPMC11302795

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.