Evidence map›Paper›PMID 39111172›Full record

ArticleTranslational oncology2024

Glutamine withdrawal leads to the preferential activation of lipid metabolism in metastatic colorectal cancer.

Aliye Ezgi Güleç Taşkıran, Diren Arda Karaoğlu, Cemil Can Eylem, Çağdaş Ermiş, İsmail Güderer, Emirhan Nemutlu, Seçil Demirkol Canlı, Sreeparna Banerjee

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Article in Translational oncology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Review
  4. Article
  5. Review
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  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Aliye Ezgi Güleç TaşkıranDepartment of Biological Sciences, Orta Doğu Teknik Üniversitesi, Ankara, Türkiye; Department of Molecular Biology and Genetics, Başkent University, Ankara, Türkiye.
Diren Arda KaraoğluFaculty of Medicine, Hacettepe University, Ankara, Türkiye.
Cemil Can EylemDepartment of Analytical Chemistry, Faculty of Pharmacy, Hacettepe University, Ankara, Türkiye.
Çağdaş ErmişDepartment of Biological Sciences, Orta Doğu Teknik Üniversitesi, Ankara, Türkiye.
İsmail GüdererDepartment of Biological Sciences, Orta Doğu Teknik Üniversitesi, Ankara, Türkiye.
Emirhan NemutluDepartment of Analytical Chemistry, Faculty of Pharmacy, Hacettepe University, Ankara, Türkiye.
Seçil Demirkol CanlıDivision of Tumor Pathology, Department of Clinical Oncology, Cancer Institute, Hacettepe University, Ankara, Türkiye.
Sreeparna BanerjeeDepartment of Biological Sciences, Orta Doğu Teknik Üniversitesi, Ankara, Türkiye; Cancer Systems Biology (CanSyL), Orta Doğu Teknik Üniversitesi, Ankara, Türkiye. Electronic address: banerjee@metu.edu.tr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionGlutamine is a non-essential amino acid that is critical for cell growth. However, the differential metabolism of l-glutamine in metastatic versus primary colorectal cancer (CRC) has not been evaluated adequately. MATERIALS AND

methodsDifferential expression of glutamine-related genes was determined in primary versus metastatic CRC. Univariate Cox regression and hierarchical clustering were used to generate a gene signature for prognostication. Untargeted metabolomics and

resultsThe GO term "Glutamine metabolism" was significantly enriched in metastatic versus primary tumors. Supporting this, SW620 cells showed decreased membrane localization of E-cadherin and increased motility upon l-Glutamine withdrawal. A glutamine related signature associated with worse prognosis was identified and validated in multiple datasets. A fluxomics assay revealed a slower TCA cycle in SW480 and SW620 cells upon l-Glutamine withdrawal. SW620 cells, however, could maintain high ATP levels. Untargeted metabolomics indicated the preferential metabolism of fatty acids in SW620 but not SW480 cells. Lipids were mainly obtained from the environment rather than by de novo synthesis.

conclusionsMetastatic CRC cells can display aberrant glutamine metabolism. We show for the first time that upon l-glutamine withdrawal, SW620 (but not SW480) cells were metabolically plastic and could metabolize lipids for survival and cellular motility.

Indexed as

Colorectal cancerL-GlutamineLipid metabolismMetabolic plasticity

Identifiers

PMID39111172
PMCPMC11362781

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.