Evidence map›Paper›PMID 39116185›Full record

ArticlePLoS pathogens2024

HIV transcription persists in the brain of virally suppressed people with HIV.

Janna Jamal Eddine, Thomas A Angelovich, Jingling Zhou, Sarah J Byrnes, Carolin Tumpach, Nadia Saraya, Emily Chalmers, Rory A Shepherd, Abigail Tan, Stephanie Marinis and 7 more

Abstract read
In one paragraph

Article in PLoS pathogens, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Janna Jamal EddineInfectious and Inflammatory Diseases, School of Health and Biomedical Sciences, RMIT University; Melbourne, Australia.
Thomas A AngelovichInfectious and Inflammatory Diseases, School of Health and Biomedical Sciences, RMIT University; Melbourne, Australia.
Jingling ZhouInfectious and Inflammatory Diseases, School of Health and Biomedical Sciences, RMIT University; Melbourne, Australia.
Sarah J ByrnesInfectious and Inflammatory Diseases, School of Health and Biomedical Sciences, RMIT University; Melbourne, Australia.
Carolin TumpachDepartment of Infectious Diseases, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
Nadia SarayaDepartment of Infectious Diseases, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
Emily ChalmersInfectious and Inflammatory Diseases, School of Health and Biomedical Sciences, RMIT University; Melbourne, Australia.
Rory A ShepherdDepartment of Infectious Diseases, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
Abigail TanDepartment of Infectious Diseases, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
Stephanie MarinisInfectious and Inflammatory Diseases, School of Health and Biomedical Sciences, RMIT University; Melbourne, Australia.
Paul R GorryDepartment of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
Jacob D EstesInfectious and Inflammatory Diseases, School of Health and Biomedical Sciences, RMIT University; Melbourne, Australia.
Bruce J BrewPeter Duncan Neurosciences Unit, Departments of Neurology and Immunology St Vincent's Hospital, Sydney, University of New South Wales and University of Notre Dame; Sydney, Australia.
Sharon R LewinDepartment of Infectious Diseases, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
Sushama TelwatteDepartment of Infectious Diseases, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.
Michael RocheInfectious and Inflammatory Diseases, School of Health and Biomedical Sciences, RMIT University; Melbourne, Australia.
Melissa J ChurchillInfectious and Inflammatory Diseases, School of Health and Biomedical Sciences, RMIT University; Melbourne, Australia.ORCID 0000-0001-5510-7954

Funding

Characterization of the intact and defective HIV reservoirs in myeloid cells in the brainR21DA055489 · NIDA · ROYAL MELBOURNE INSTITUTE OF TECHNOLOGY · PI ANGELOVICH, THOMAS A, CHURCHILL, MELISSA · 2021 to 2022
$272k
NIDA NIH HHS R21 DA055489
6 · The paper itself

Abstract

HIV persistence in the brain is a barrier to cure, and potentially contributes to HIV-associated neurocognitive disorders. Whether HIV transcription persists in the brain despite viral suppression with antiretroviral therapy (ART) and is subject to the same blocks to transcription seen in other tissues and blood, is unclear. Here, we quantified the level of HIV transcripts in frontal cortex tissue from virally suppressed or non-virally suppressed people with HIV (PWH). HIV transcriptional profiling of frontal cortex brain tissue (and PBMCs where available) from virally suppressed (n = 11) and non-virally suppressed PWH (n = 13) was performed using digital polymerase chain reaction assays (dPCR). CD68+ myeloid cells or CD3+ T cells expressing HIV p24 protein present in frontal cortex tissue was detected using multiplex immunofluorescence imaging. Frontal cortex brain tissue from PWH had HIV TAR (n = 23/24) and Long-LTR (n = 20/24) transcripts. Completion of HIV transcription was evident in brain tissue from 12/13 non-virally suppressed PWH and from 5/11 virally suppressed PWH, with HIV p24+CD68+ cells detected in these individuals. While a block to proximal elongation was present in frontal cortex tissue from both PWH groups, this block was more extensive in virally suppressed PWH. These findings suggest that the brain is a transcriptionally active HIV reservoir in a subset of virally suppressed PWH.

Indexed as

BrainHIV-1HIV InfectionsAdultFemaleFrontal LobeHumansMaleMiddle AgedTranscription, Genetic

Identifiers

PMID39116185
PMCPMC11335163

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.