Evidence map›Paper›PMID 39118286›Full record

ArticleClinical and translational medicine2024

Macrophage OTUD1-CARD9 axis drives isoproterenol-induced inflammatory heart remodelling.

Jinfu Qian, Qinyan Wang, Jiachen Xu, Shiqi Liang, Qingsong Zheng, Xiaocheng Guo, Wu Luo, Weijian Huang, Xiaohong Long, Julian Min and 3 more

Abstract read
In one paragraph

Article in Clinical and translational medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Jinfu QianDepartment of Cardiology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Qinyan WangChemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
Jiachen XuDepartment of Cardiology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Shiqi LiangChemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
Qingsong ZhengChemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
Xiaocheng GuoChemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
Wu LuoDepartment of Cardiology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Weijian HuangDepartment of Cardiology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Xiaohong LongChemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
Julian MinChemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
Yi WangChemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
Gaojun WuDepartment of Cardiology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Guang LiangDepartment of Cardiology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.ORCID 0000-0002-8278-849X

Funding

National Natural Science Foundation of China 81930108National Natural Science Foundation of China 82271347Wenzhou City Research Project ZY2020016
6 · The paper itself

Abstract

backgroundChronic inflammation contributes to the progression of isoproterenol (ISO)-induced heart failure (HF). Caspase-associated recruitment domain (CARD) families are crucial proteins for initiation of inflammation in innate immunity. Nonetheless, the relevance of CARDs in ISO-driven cardiac remodelling is little explored.

methodsThis study utilized Card9

resultsHere, we demonstrated that CARD9 was substantially upregulated in murine hearts infused with ISO. Either whole-body CARD9 knockout or myeloid-specific CARD9 deletion inhibited ISO-driven murine cardiac inflammation, remodelling and dysfunction. CARD9 deficiency in macrophages prevented ISO-induced inflammation and alleviated remodelling changes in cardiomyocytes and fibroblasts. Mechanistically, we found that ISO enhances the activity of CARD9 by upregulating ovarian tumour deubiquitinase 1 (OTUD1) in macrophages. We further demonstrated that OTUD1 directly binds to the CARD9 and then removes the K33-linked ubiquitin from CARD9 to promote the assembly of the CARD9-BCL10-MALT1 (CBM) complex, without affecting CARD9 stability. The ISO-activated CBM complex results in NF-κB activation and macrophage-based inflammatory gene overproduction, which then enhances cardiomyocyte hypertrophy and fibroblast fibrosis, respectively. Myeloid-specific OTUD1 deletion also attenuated ISO-induced murine cardiac inflammation and remodelling.

conclusionsThese results suggested that the OTUD1-CARD9 axis is a new pro-inflammatory signal in ISO-challenged macrophages and targeting this axis has a protective effect against ISO-induced HF. KEY POINTS: Macrophage CARD9 was elevated in heart tissues of mice under chronic ISO administration. Either whole-body CARD9 knockout or myeloid-specific CARD9 deficiency protected mice from ISO-induced inflammatory heart remodeling. ISO promoted the assembly of CBM complex and then activated NF-κB signaling in macrophages through OTUD1-mediated deubiquitinating modification. OTUD1 deletion in myeloid cells protected hearts from ISO-induced injuries in mice.

Indexed as

CARD Signaling Adaptor ProteinsIsoproterenolMacrophagesAnimalsDisease Models, AnimalHumansInflammationMiceMice, Inbred C57BLMice, KnockoutMyocytes, CardiacUbiquitin-Specific ProteasesVentricular RemodelingCard9 protein, mouseCARD Signaling Adaptor ProteinsIsoproterenolUbiquitin-Specific ProteasesCARD9heart failureinflammationisoproterenolmacrophageOTUD1

Identifiers

PMID39118286
PMCPMC11310286

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.