Evidence map›Paper›PMID 39138165›Full record

ArticleNature communications2024

Cellular transitions during cranial suture establishment in zebrafish.

D'Juan T Farmer, Jennifer E Dukov, Hung-Jhen Chen, Claire Arata, Jose Hernandez-Trejo, Pengfei Xu, Camilla S Teng, Robert E Maxson, J Gage Crump

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

D'Juan T FarmerDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, 90095, USA. djuanfar@mcdb.ucla.edu.ORCID 0000-0002-3636-6835
Jennifer E DukovDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, 90095, USA.
Hung-Jhen ChenDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, 90095, USA.ORCID 0000-0003-1714-3596
Claire ArataEli and Edythe Broad Center for Regenerative Medicine, Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, 90033, USA.ORCID 0000-0003-1535-1818
Jose Hernandez-TrejoEli and Edythe Broad Center for Regenerative Medicine, Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, 90033, USA.
Pengfei XuDepartment of Orofacial Sciences and Program in Craniofacial Biology, University of California, San Francisco, San Francisco, CA, USA.
Camilla S TengDepartment of Cell and Tissue Biology, University of California San Francisco, San Francisco, CA, 94143, USA.
Robert E MaxsonDepartment of Biochemistry, Keck School of Medicine, University of Southern California, Los Angeles, CA, 90033, USA.
J Gage CrumpEli and Edythe Broad Center for Regenerative Medicine, Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, 90033, USA. gcrump@usc.edu.ORCID 0000-0002-3209-0026

Funding

Molecular and Cellular Basis of CraniosynostosisR01DE026339 · NIDCR · UNIVERSITY OF SOUTHERN CALIFORNIA · PI CRUMP, GAGE D · 2016 to 2025
$6.5M
Training in Developmental Biology, Stem Cells and RegenerationT32HD060549 · NICHD · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Gage D Crump · 2011 to 2026
$2.5M
NICHD NIH HHS T32 HD060549NIDCR NIH HHS R01 DE026339U.S. Department of Health & Human Services | National Institutes of Health (NIH) 5R01DE026339
6 · The paper itself

Abstract

Cranial sutures separate neighboring skull bones and are sites of bone growth. A key question is how osteogenic activity is controlled to promote bone growth while preventing aberrant bone fusions during skull expansion. Using single-cell transcriptomics, lineage tracing, and mutant analysis in zebrafish, we uncover key developmental transitions regulating bone formation at sutures during skull expansion. In particular, we identify a subpopulation of mesenchyme cells in the mid-suture region that upregulate a suite of genes including BMP antagonists (e.g. grem1a) and pro-angiogenic factors. Lineage tracing with grem1a:nlsEOS reveals that this mid-suture subpopulation is largely non-osteogenic. Moreover, combinatorial mutation of BMP antagonists enriched in this mid-suture subpopulation results in increased BMP signaling in the suture, misregulated bone formation, and abnormal suture morphology. These data reveal establishment of a non-osteogenic mesenchyme population in the mid-suture region that restricts bone formation through local BMP antagonism, thus ensuring proper suture morphology.

Indexed as

Bone Morphogenetic ProteinsCranial SuturesMesodermOsteogenesisZebrafishZebrafish ProteinsAnimalsGene Expression Regulation, DevelopmentalMutationSignal TransductionSingle-Cell AnalysisSkullBone Morphogenetic ProteinsZebrafish Proteins

Identifiers

PMID39138165
PMCPMC11322166

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.