Evidence map›Paper›PMID 39138923›Full record

ArticleJournal of cellular physiology2024

Quantitative assessment of morphological changes in lipid droplets and lipid-mito interactions with aging in brown adipose.

Amber Crabtree, Kit Neikirk, Julia A Pinette, Aaron Whiteside, Bryanna Shao, Jessica Bedenbaugh, Zer Vue, Larry Vang, Han Le, Mert Demirci and 26 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular physiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Cold exposure-inducedActa pharmaceutica Sinica. B · 2026
    Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

36 authors.

Amber CrabtreeDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Kit NeikirkDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Julia A PinetteDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Aaron WhitesideDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Bryanna ShaoDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Jessica BedenbaughDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Zer VueDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Larry VangDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Han LeDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Mert DemirciDepartment of Medicine, Division Nephrology and Hypertension, Vanderbilt University Medical Center, Nashville, Tennessee, USA.
Taseer AhmadDepartment of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee, USA.
Trinity Celeste OwensDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Ashton OliverDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Faben ZelekeDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Heather K BeasleyDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Edgar Garza LopezDepartment of Internal Medicine, University of Iowa, Iowa City, Iowa, USA.
Estevão ScudeseDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Taylor RodmanDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Kinuthia KabugiDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Alice KohDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Suzanne NavarroDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Jacob LamDepartment of Internal Medicine, University of Iowa, Iowa City, Iowa, USA.
Ben KirkDepartment of Internal Medicine, University of Iowa, Iowa City, Iowa, USA.
Margaret MungaiDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Mariya SweetwyneDepartment of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, USA.
Ho-Jin KohDepartment of Biological Sciences, Tennessee State University, Nashville, Tennessee, USA.
Elma ZaganjorDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Steven M DamoDepartment of Life and Physical Sciences, Fisk University, Nashville, Tennessee, USA.
Jennifer A GaddyDivision of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
Annet KiraboDepartment of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee, USA.ORCID 0000-0001-8580-9359
Sandra A MurrayDepartment of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Anthonya CooperDepartment of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Clintoria WilliamsDepartment of Neuroscience, Cell Biology and Physiology, Wright State University, Dayton, Ohio, USA.
Melanie R McReynoldsDepartment of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania, USA.
Andrea G MarshallDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Antentor HintonDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.ORCID 0000-0002-7730-952X

Funding

ENaC regulation and its role in blood pressure homeostasisR01HL147818 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · 2022 to 2025
$2.0M
Salt Mediated Cross Talk Between Lymphatic Vessels and Immune Cells in Kidney DiseaseR01DK135764 · VANDERBILT UNIVERSITY MEDICAL CENTER · 2025 to 2025
$734k
Immune Mechanisms of Salt-Sensitive hypertensionR01HL144941 · VANDERBILT UNIVERSITY MEDICAL CENTER · 2025 to 2025
$572k
Deep phenotypic and functional characterization of salt-responsive immune cells in human salt senstive hypertension using CTE-seqR03HL155041 · NHLBI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Annet Kirabo Kirabo · 2022 to 2022
$87k
Chan Zuckerberg Initiative DAF 2022-253529Chan Zuckerberg Initiative DAF 2022-253614Department of Veterans Affairs Office of Research M.T.S. I01 BX005352FIC NIH HHS R21 TW012635Multidisciplinary Training in Molecular Endocrinology DK007563NHLBI NIH HHS R01 HL144941NHLBI NIH HHS R01 HL147818NHLBI NIH HHS R01 HL157584NHLBI NIH HHS R03 HL155041NIDDK NIH HHS R01 DK135764NIH HHS K01AG062757NIH HHS K01HL130497NIH HHS R01HL144941NIH HHS R01HL147818NIH HHS R03HL155041NSF EES1817282NSF EES2112556NSF MCB1955975NSF MCB #2011577INSF BPE 22-17621(K.Stassun,PI)NSF NRT 19-22697(K.Stassun,PI)
6 · The paper itself

Abstract

The physical characteristics of brown adipose tissue (BAT) are defined by the presence of multilocular lipid droplets (LDs) within the brown adipocytes and a high abundance of iron-containing mitochondria, which give it its characteristic color. Normal mitochondrial function is, in part, regulated by organelle-to-organelle contacts. For example, the contact sites that mediate mitochondria-LD interactions are thought to have various physiological roles, such as the synthesis and metabolism of lipids. Aging is associated with mitochondrial dysfunction, and previous studies show that there are changes in mitochondrial structure and the proteins that modulate organelle contact sites. However, how mitochondria-LD interactions change with aging has yet to be fully clarified. Therefore, we sought to define age-related changes in LD morphology and mitochondria-lipid interactions in BAT. We examined the three-dimensional morphology of mitochondria and LDs in young (3-month) and aged (2-year) murine BAT using serial block face-scanning electron microscopy and the Amira program for segmentation, analysis, and quantification. Our analyses showed reductions in LD volume, area, and perimeter in aged samples in comparison to young samples. Additionally, we observed changes in LD appearance and type in aged samples compared to young samples. Notably, we found differences in mitochondrial interactions with LDs, which could implicate that these contacts may be important for energetics in aging. Upon further investigation, we also found changes in mitochondrial and cristae structure for the mitochondria interacting with LDs. Overall, these data define the nature of LD morphology and organelle-organelle contacts during aging and provide insight into LD contact site changes that interconnect biogerontology with mitochondrial function, metabolism, and bioactivity in aged BAT.

Indexed as

Adipose Tissue, BrownAgingLipid DropletsMitochondriaAdipocytes, BrownAnimalsLipid MetabolismMaleMiceMice, Inbred C57BLbrown adipose tissue (BAT)lipid droplets (LD)lipidsmitochondriamito–lipid

Identifiers

PMID39138923
PMCPMC13258579

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.