Evidence map›Paper›PMID 39143258›Full record

ArticleEMBO reports2024

Satellite cell-derived TRIM28 is pivotal for mechanical load- and injury-induced myogenesis.

Kuan-Hung Lin, Jamie E Hibbert, Corey Gk Flynn, Jake L Lemens, Melissa M Torbey, Nathaniel D Steinert, Philip M Flejsierowicz, Kiley M Melka, Garrison T Lindley, Marcos Lares and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EMBO reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Making sense of MYC in skeletal muscle: location, duration, and magnitude.American journal of physiology. Cell physiology · 2025
    Article
  2. Review
  3. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Kuan-Hung Lin *Department of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA.
Jamie E Hibbert *Department of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA.
Corey Gk FlynnDepartment of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA.
Jake L LemensDepartment of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA.
Melissa M TorbeyDepartment of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA.ORCID 0009-0005-3984-9049
Nathaniel D SteinertDepartment of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA.
Philip M FlejsierowiczDepartment of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA.
Kiley M MelkaDepartment of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA.
Garrison T LindleyDepartment of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA.
Marcos LaresDepartment of Dermatology, University of Wisconsin - Madison, Madison, WI, USA.
Vijayasaradhi SetaluriDepartment of Dermatology, University of Wisconsin - Madison, Madison, WI, USA.
Amy J WagersDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.ORCID 0000-0002-0066-0485
Troy A HornbergerDepartment of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA. troy.hornberger@wisc.edu.ORCID 0000-0002-2349-1899

Funding

Skin Diseases Research Center at the University of WisconsinP30AR066524 · NIAMS · UNIVERSITY OF WISCONSIN-MADISON · PI LONGLEY, B JACK · 2014 to 2018
$2.6M
The Role of TRIM28 Phosphorylation in the Mechanical Regulation of Skeletal Muscle - Re-entry SupplementR01AR074932 · NIAMS · UNIVERSITY OF WISCONSIN-MADISON · PI HORNBERGER, TROY A · 2020 to 2024
$2.0M
Identifying the Structural Adaptations that Drive the Mechanically Induced Growth of Skeletal MuscleR01AR082816 · NIAMS · UNIVERSITY OF WISCONSIN-MADISON · PI TROY A HORNBERGER · 2023 to 2026
$1.9M
BLRD VA IK6 BX006317HHS | NIH | National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) AR074932HHS | NIH | National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) AR074932-S1HHS | NIH | National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) P30AR066524NIAMS NIH HHS R01 AR074932NIAMS NIH HHS R01 AR082816
6 · The paper itself

Abstract

Satellite cells are skeletal muscle stem cells that contribute to postnatal muscle growth, and they endow skeletal muscle with the ability to regenerate after a severe injury. Here we discover that this myogenic potential of satellite cells requires a protein called tripartite motif-containing 28 (TRIM28). Interestingly, different from the role reported in a previous study based on C2C12 myoblasts, multiple lines of both in vitro and in vivo evidence reveal that the myogenic function of TRIM28 is not dependent on changes in the phosphorylation of its serine 473 residue. Moreover, the functions of TRIM28 are not mediated through the regulation of satellite cell proliferation or differentiation. Instead, our findings indicate that TRIM28 regulates the ability of satellite cells to progress through the process of fusion. Specifically, we discover that TRIM28 controls the expression of a fusogenic protein called myomixer and concomitant fusion pore formation. Collectively, the outcomes of this study expose the framework of a novel regulatory pathway that is essential for myogenesis.

Indexed as

Muscle DevelopmentSatellite Cells, Skeletal MuscleTripartite Motif-Containing Protein 28AnimalsCell DifferentiationCell FusionCell LineCell ProliferationHumansMiceMuscle, SkeletalPhosphorylationTRIM28 protein, humanTrim28 protein, mouseTripartite Motif-Containing Protein 28Cell FusionHypertrophyRegenerationSkeletal Muscle

Identifiers

PMID39143258
PMCPMC11387408

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.