Evidence map›Paper›PMID 39150597›Full record

ArticleFish physiology and biochemistry2024

PI3KR1 and AKT1 in largemouth bass (Micropterus salmoides): molecular cloning, characterization, and its involvement in the alleviation of hepatic glycogen deposition caused by insulin inclusion in vitro.

Yuru Li, Shiwen Chen, Yijun Liu, Pingping Liu, Songlin Li, Ning Liu

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Article in Fish physiology and biochemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Yuru LiInternational Research Centre for Food and Health, College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.
Shiwen ChenResearch Centre of the Ministry of Agriculture and Rural Affairs On Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, China.
Yijun LiuResearch Centre of the Ministry of Agriculture and Rural Affairs On Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, China.
Pingping LiuInternational Research Centre for Food and Health, College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.
Songlin LiResearch Centre of the Ministry of Agriculture and Rural Affairs On Environmental Ecology and Fish Nutrition, Shanghai Ocean University, Shanghai, 201306, China. slli@shou.edu.cn.
Ning LiuInternational Research Centre for Food and Health, College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China. nliu@shou.edu.cn.

Funding

National Natural Science Foundation of China 31802308Natural Science Foundation of Shanghai No. 21ZR1427300
6 · The paper itself

Abstract

In this study, the full-length cDNA sequences of the phosphatidylinositol-3-kinase p85 alpha (PI3KR1) and serine/threonine kinase 1 (AKT1) genes in largemouth bass (Micropterus salmoides) were obtained using the rapid amplification of cDNA ends (RACE) method. Sequence analysis revealed that the cloned sequences of PI3KR1 and AKT1 are 4170 bp and 3672 bp in length, with open reading frames (ORFs) of 1389 bp and 1422 bp encoding 462 and 473 amino acids, respectively. Sequence alignment and evolutionary tree analysis indicated their close relationship to other teleosts, especially those with similar feeding habits. Tissue distribution demonstrated widespread distribution of both genes in various tissues, with the highest abundance in the liver. Further results found that the upregulation of the expression of p-PI3KR1, p-AKT1, p-FoxO1, and GLUT2 proteins by insulin, while suppressing the expression of the total FoxO1 protein, effectively triggers a significant activation of the PI3KR1-AKT1 insulin signaling pathway. Meanwhile, the mRNA levels of the key glycolytic genes, including glucokinase (gk), pyruvate kinase (pk), and phosphofructokinase liver type (pfkl), have been enhanced evidently. In contrast, the expression of gluconeogenic genes such as phosphoenolpyruvate carboxykinase (pepck), glucose-6-phosphatase catalytic subunit (g6pc), and fructose-1,6-bisphosphatase-1 (fbp1) has been notably down-regulated. In addition, insulin treatment promoted the phosphorylation of glycogen phosphorylase (PYGL) and the dephosphorylation of glycogen synthase (GS), and the glycogen content in the insulin-treated group was remarkably reduced compared to the control group. Overall, our study indicates that the activation of PI3KR1-AKT1 insulin signaling pathway represses the hepatic glycogen deposition via the regulation of glycolysis and gluconeogenesis, which provides some new insights into nutritional strategy to effectively regulate the glucose metabolism in carnivorous fish.

Indexed as

BassCloning, MolecularInsulinProto-Oncogene Proteins c-aktAmino Acid SequenceAnimalsFish ProteinsLiverLiver GlycogenPhylogenyFish ProteinsInsulinLiver GlycogenProto-Oncogene Proteins c-aktGene expressionGlycogen accumulationInsulinLargemouth bassMolecular cloning

Identifiers

PMID39150597

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.