Evidence map›Paper›PMID 39154310›Full record

ArticleMolecular biology reports2024

Positive cooperativity in synergistic activation of Wnt proteins.

Clemence Bonnet, Christiana S Han, Sophie X Deng, Jie J Zheng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Clemence BonnetStein Eye Institute, University of California, Los Angeles, CA, 90095, USA.ORCID http://orcid.org/0000-0003-1732-7643
Christiana S HanStein Eye Institute, University of California, Los Angeles, CA, 90095, USA.
Sophie X DengStein Eye Institute, University of California, Los Angeles, CA, 90095, USA.
Jie J ZhengStein Eye Institute, University of California, Los Angeles, CA, 90095, USA. jzheng@jsei.ucla.edu.

Funding

Vision Research Core at UCLAP30EY000331 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DAVID S WILLIAMS · 1985 to 2026
$16.8M
Ex Vivo Expansion of Human Limbal Stem Cells for TransplantationR01EY021797 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Sophie Deng · 2012 to 2026
$5.1M
Development of small-molecule Wnt mimetics for corneal epithelial cell regenerationR01EY028557 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DENG, SOPHIE, ZHENG, JIE J. · 2018 to 2021
$2.0M
California Institute for Regenerative Medicine TR2-01768NEI NIH HHS 5P30EY000331NEI NIH HHS P30 EY000331NEI NIH HHS R01EY021797NEI NIH HHS R01 EY028557NEI NIH HHS R01EY028557
6 · The paper itself

Abstract

backgroundWnt proteins are crucial for embryonic development, stem cell growth, and tissue regeneration. Wnt signaling pathway is activated when Wnt proteins bind to cell membrane receptors. METHODS AND

resultsWe employed a luciferase reporter assay in HEK293STF cells to measure Wnt protein-induced signaling. We observed that Wnt3a uniquely promotes the Wnt/β-catenin pathway through positive cooperativity. Additionally, MFH-ND, a molecular mimic of Wnt ligands, markedly increased Wnt3a-induced signaling in a dose-responsive manner. This suggests that various Wnt ligands can synergistically enhance Wnt pathway activation.

conclusionsThe study suggests the likelihood of various Wnt ligands coexisting in a single signalosome on the cell membrane, providing new insights into the complexities of Wnt signaling mechanisms.

Indexed as

Wnt3A ProteinWnt Signaling Pathwaybeta CateninHEK293 CellsHumansLigandsWnt Proteinsbeta CateninLigandsWnt3A ProteinWNT3A protein, humanWnt ProteinsCooperative bindingSignalosomeWnt3aWnt/b-catenin signalingWnt mimic

Identifiers

PMID39154310
PMCPMC11330945

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.