Evidence map›Paper›PMID 39158740›Full record

ArticleMolecular biology reports2024

GM130-silencing may aggravate blood-brain barrier damage and affect microglia polarization by down-regulating PD-L1 expression after experimental intracerebral hemorrhage.

Xiqian Chen, Yijun Ren, Pinghui Xie, Qiang Lei, Wei Lu

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
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1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Xiqian Chen *Department of Neurology, Second Xiangya Hospital, Central South University, No. 139, Renmin Middle Road, Changsha, Hunan, China.
Yijun Ren *Department of Neurology, Second Xiangya Hospital, Central South University, No. 139, Renmin Middle Road, Changsha, Hunan, China.
Pinghui XieDepartment of Neurology, Changsha Central Hospital, Changsha, Hunan, China.
Qiang LeiDepartment of Neurology, Second Xiangya Hospital, Central South University, No. 139, Renmin Middle Road, Changsha, Hunan, China.
Wei LuDepartment of Neurology, Second Xiangya Hospital, Central South University, No. 139, Renmin Middle Road, Changsha, Hunan, China. luwei0338@csu.edu.cn.

Funding

This work was funded by the National Natural Science Foundation of China #81571181
6 · The paper itself

Abstract

backgroundIn addition to primary injury, secondary injuries related to BBB disruption and immune-inflammatory response also play an important role in intracerebral hemorrhage (ICH). And the Golgi apparatus play an important role in the state of ICH.

methodsICH model and GM130-silencing ICH model were established in SD rats. The Garcia score was used to score the neurological defects of the rats. Blood-brain barrier (BBB) integrity were assessed by amount of extravasated Evans blue, and tight junction proteins. The expression of PD-L1 and GM130were detected through Western-blot and the subtype of microglia was showing with Immunofluorescence staining.

resultsCompared with the ICH group, GM130-silencing ICH rats got a worsened neurological deficit and enlarged volume of the hematoma. Evan's blue extravasation aggravated as well. The expression of GM130 in peri-hematoma tissue was further decreased, and the morphology and structure of the Golgi apparatus were further damaged. Meanwhile, the GM130 deficit resulted in decreased expression of PD-L1 and more polarization of microglia to the M1 subtype.

conclusionWe demonstrate that GM130 could influence the integrity of BBB and plays a role in neuroinflammation via regulation of PD-L1 after ICH. The manipulation of GM130 might be a promising therapeutical target in ICH.

Indexed as

B7-H1 AntigenBlood-Brain BarrierCerebral HemorrhageMembrane ProteinsMicrogliaAnimalsAutoantigensDisease Models, AnimalDown-RegulationGolgi ApparatusMaleRatsRats, Sprague-DawleyAutoantigensB7-H1 AntigenGolgin subfamily A member 2Membrane ProteinsGM130Intracerebral hemorrhageMicroglia polarizationPD-L1

Identifiers

PMID39158740

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.