Evidence map›Paper›PMID 39164392›Full record

ArticleNature microbiology2024

Gut epithelial electrical cues drive differential localization of enterobacteria.

Yaohui Sun, Fernando Ferreira, Brian Reid, Kan Zhu, Li Ma, Briana M Young, Catherine E Hagan, Renée M Tsolis, Alex Mogilner, Min Zhao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yaohui Sun *Department of Ophthalmology and Vision Science, Department of Dermatology, School of Medicine, University of California, Davis, Sacramento, CA, USA. yhsun@ucdavis.edu.ORCID http://orcid.org/0000-0002-3716-6659
Fernando Ferreira *Department of Ophthalmology and Vision Science, Department of Dermatology, School of Medicine, University of California, Davis, Sacramento, CA, USA.ORCID http://orcid.org/0000-0001-5786-8896
Brian ReidDepartment of Ophthalmology and Vision Science, Department of Dermatology, School of Medicine, University of California, Davis, Sacramento, CA, USA.
Kan ZhuDepartment of Ophthalmology and Vision Science, Department of Dermatology, School of Medicine, University of California, Davis, Sacramento, CA, USA.ORCID http://orcid.org/0000-0002-1146-8130
Li MaDepartment of Ophthalmology and Vision Science, Department of Dermatology, School of Medicine, University of California, Davis, Sacramento, CA, USA.
Briana M YoungDepartment of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, Davis, CA, USA.
Catherine E HaganDepartment of Comparative Medicine, School of Medicine, University of Washington, Seattle, WA, USA.
Renée M TsolisDepartment of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, Davis, CA, USA.ORCID http://orcid.org/0000-0001-9131-6657
Alex MogilnerCourant Institute and Department of Biology, New York University, New York, NY, USA. mogilner@cims.nyu.edu.ORCID http://orcid.org/0000-0002-9310-3812
Min ZhaoDepartment of Ophthalmology and Vision Science, Department of Dermatology, School of Medicine, University of California, Davis, Sacramento, CA, USA. minzhao@ucdavis.edu.ORCID http://orcid.org/0000-0002-2500-3035

Funding

VISION RESEARCH CORE GRANTP30EY012576 · NEI · UNIVERSITY OF CALIFORNIA DAVIS · PI MARIE E BURNS · 1999 to 2026
$18.9M
Molecular Generators at Corneal Wounds Produce and regulate the Wound Electrical SignalsR01EY019101 · NEI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI ZHAO, MIN · 2010 to 2023
$5.2M
Bioelectricity in Gut Epithelium Drives Pathogenic Bacterial TargetingR21AI156409 · NIAID · UNIVERSITY OF CALIFORNIA AT DAVIS · PI SUN, YAOHUI, ZHAO, MIN · 2021 to 2022
$432k
Division of Intramural Research, National Institute of Allergy and Infectious Diseases (Division of Intramural Research of the NIAID) 1R21AI156409NEI NIH HHS P30 EY012576NEI NIH HHS R01 EY019101NIAID NIH HHS R21 AI156409United States Department of Defense | Defense Advanced Research Projects Agency (DARPA) HR001119S0027
6 · The paper itself

Abstract

Salmonella translocate to the gut epithelium via microfold cells lining the follicle-associated epithelium (FAE). How Salmonella localize to the FAE is not well characterized. Here we use live imaging and competitive assays between wild-type and chemotaxis-deficient mutants to show that Salmonella enterica serotype Typhimurium (S. Typhimurium) localize to the FAE independently of chemotaxis in an ex vivo mouse caecum infection model. Electrical recordings revealed polarized FAE with sustained outward current and small transepithelial potential, while the surrounding villus is depolarized with inward current and large transepithelial potential. The distinct electrical potentials attracted S. Typhimurium to the FAE while Escherichia coli (E. coli) localized to the villi, through a process called galvanotaxis. Chloride flux involving the cystic fibrosis transmembrane conductance regulator (CFTR) generated the ionic currents around the FAE. Pharmacological inhibition of CFTR decreased S. Typhimurium FAE localization but increased E. coli recruitment. Altogether, our findings demonstrate that bioelectric cues contribute to S. Typhimurium targeting of specific gut epithelial locations, with potential implications for other enteric bacterial infections.

Indexed as

Cystic Fibrosis Transmembrane Conductance RegulatorEscherichia coliIntestinal MucosaSalmonella typhimuriumAnimalsCecumChemotaxisDisease Models, AnimalFemaleHumansMiceMice, Inbred C57BLCystic Fibrosis Transmembrane Conductance Regulator

Identifiers

PMID39164392
PMCPMC11445056

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.