Evidence map›Paper›PMID 39177836›Full record

ArticleArchives of microbiology2024

Integrative workflows for the characterization of hydrophobin and cerato-platanin in the marine fungus Paradendryphiella salina.

Catalina Landeta, David Medina-Ortiz, Natalia Escobar, Iván Valdez, María Paz González-Troncoso, Diego Álvares-Saravia, Jacqueline Aldridge, Carlos Gómez, María Elena Lienqueo

Abstract read
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In one paragraph

Article in Archives of microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Catalina LandetaCenter for Biotechnology and Bioengineering (CeBiB), Department of Chemical Engineering, Biotechnology, and Materials, Faculty of Physical and Mathematical Sciences, University of Chile, Santiago, Beauchef, 851- 8370456, Chile.
David Medina-OrtizCenter for Biotechnology and Bioengineering (CeBiB), Department of Chemical Engineering, Biotechnology, and Materials, Faculty of Physical and Mathematical Sciences, University of Chile, Santiago, Beauchef, 851- 8370456, Chile.
Natalia EscobarMicrobiology, Department of Biology, Utrecht University, Utrecht, Netherlands.
Iván ValdezMicrobiology, Department of Biology, Utrecht University, Utrecht, Netherlands.
María Paz González-TroncosoCenter for Biotechnology and Bioengineering (CeBiB), Department of Chemical Engineering, Biotechnology, and Materials, Faculty of Physical and Mathematical Sciences, University of Chile, Santiago, Beauchef, 851- 8370456, Chile.
Diego Álvares-SaraviaTeaching and Research Assistance Center, CADI, University of Magallanes, Av. los Flamencos, Punta Arenas, 01364, Chile.
Jacqueline AldridgeDepartment of Computer Engineering, Faculty of Engineering, University of Magallanes, Av. Pdte. Manuel Bulnes 01855, Punta Arenas, Chile.
Carlos GómezChemistry Department, University of Valle-Yumbo, Valle del Cauca, 760501, Chile.
María Elena LienqueoCenter for Biotechnology and Bioengineering (CeBiB), Department of Chemical Engineering, Biotechnology, and Materials, Faculty of Physical and Mathematical Sciences, University of Chile, Santiago, Beauchef, 851- 8370456, Chile. mlienque@uchile.cl.

Funding

National Research and Development Agency of Chile of the Government of Chile - ANID 21161794National Research and Development Agency of Chile of the Government of Chile - ANID Centre for Biotechnology and Bioengineering - CeBiB - (Project FB0001)
6 · The paper itself

Abstract

Hydrophobins (HFBs) and cerato-platanins (CPs) are surface-active extracellular proteins produced by filamentous fungi. This study identified two HFB genes (pshyd1 and pshyd2) and one CP gene (pscp) in the marine fungus Paradendryphiella salina. The proteins PsCP, PsHYD2, and PsHYD1 had molecular weights of 12.70, 6.62, and 5.98 kDa, respectively, with isoelectric points below 7. PsHYD1 and PsHYD2 showed hydrophobicity (GRAVY score 0.462), while PsCP was hydrophilic (GRAVY score - 0.202). Stability indices indicated in-solution stability. Mass spectrometry identified 2,922 proteins, including CP but not HFB proteins. qPCR revealed differential gene expression influenced by developmental stage and substrate, with pshyd1 consistently expressed. These findings suggest P. salina's adaptation to marine ecosystems with fewer hydrophobin genes than other fungi but capable of producing surface-active proteins from seaweed carbohydrates. These proteins have potential applications in medical biocoatings, food industry foam stabilizers, and environmental bioremediation.

Indexed as

Fungal ProteinsHydrophobic and Hydrophilic InteractionsAquatic OrganismsAscomycotaGene Expression Regulation, FungalSeawaterSeaweedFungal ProteinsCerato-plataninHydrophobinMarine fungiParadendryphiella salina

Identifiers

PMID39177836

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.