ArticleJournal of assisted reproduction and genetics2024
Mitochondrial DNA content and methylation in sperm of patients with asthenozoospermia.
Article in Journal of assisted reproduction and genetics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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Who cites it
5 citing papers in PubMed.
- Sperm mitochondrial DNA copy number: a marker of male fertility and reproductive success.Human reproduction update · 2026Review
- Mitochondrial DNA methylation: State-of-the-art in molecular mechanisms and disease implications.Journal of advanced research · 2026Review
- Epigenetic effects of Qixiong Formula on sperm DNA methylation in a rat model of asthenozoospermia.Translational andrology and urology · 2025Article
- Sperm DNA methylation profiling in patients with Kallmann syndrome.Endocrine connections · 2025Article
- Protective roles of melatonin regulated mitochondrial DNA methylation and respiratory metabolism in ram sperm cryopreservation.Frontiers in veterinary science · 2025Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
purposeThe aim of the current study was to investigate the mtDNA methylation levels and mtDNA copy numbers in the sperm of patients with asthenozoospermia and compare them to those observed in controls with normozoospermia.
methodsPyrosequencing analysis of the methylation levels of the mitochondrial D-loop and MT-CO1/chr1:631,907-632083/chrX:26,471,887-126,472,063 (hereinafter referred to as "MT-CO1-AVG") region and quantitative PCR analysis of the mtDNA copy number were performed on sperm from 30 patients with asthenozoospermia and 30 controls with normozoospermia.
resultsCompared with those of controls with normozoospermia, the methylation levels of D-loop and MT-CO1-AVG regions and mtDNA copy number were significantly higher in patients with asthenozoospermia. The methylation level of the D-loop region in patients with asthenozoospermia and controls with normozoospermia and that of MT-CO1-AVG region in patients with asthenozoospermia showed a decreasing tendency with increasing total sperm motility. A significant inverse correlation between the mtDNA copy number and total sperm motility was observed in patients with asthenozoospermia but not in controls with normozoospermia. In patients with asthenozoospermia, but not in controls with normozoospermia, we observed a significant inverse correlation between D-loop methylation levels and mtDNA copy number, while no significant correlation was observed between MT-CO1-AVG methylation levels and mtDNA copy number.
conclusionThese results reveal the occurrence of mtDNA methylation in human sperm and altered D-loop and MT-CO1-AVG methylation levels in patients with asthenozoospermia. Additional research is needed to determine the function of these features in the etiology and course of asthenozoospermia.
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