Evidence mapPaperPMID 39198490Full record

ArticleScientific reports2024

Metformin enhances the therapeutic effects of extracellular vesicles derived from human periodontal ligament stem cells on periodontitis.

Mingli Xiang, Yulin Liu, Qiushuang Guo, Chengcheng Liao, Linlin Xiao, Meiling Xiang, Xiaoyan Guan, Jianguo Liu

Abstract read
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. Efficacy of metformin as an adjunct in periodontitis: A systematic analysis.Journal of Taibah University Medical Sciences · 2026
    Review
  3. Review
  4. Review
  5. Review
  6. Review
  7. Isolation methods of exosomes derived from dental stem cells.International journal of oral science · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mingli XiangGuiZhou University Medical College, Guiyang, 550025, Guizhou Province, China.
Yulin LiuOral Disease Research Key Laboratory of Guizhou Tertiary Institution, School of Stomatology, Zunyi Medical University, Zunyi, 563006, China.
Qiushuang GuoDepartment of Orthodontics II, Affiliated Stomatological Hospital of Zunyi Medical University, Zunyi, 563000, China.
Chengcheng LiaoDepartment of Orthodontics II, Affiliated Stomatological Hospital of Zunyi Medical University, Zunyi, 563000, China.
Linlin XiaoDepartment of Orthodontics II, Affiliated Stomatological Hospital of Zunyi Medical University, Zunyi, 563000, China.
Meiling XiangDepartment of Orthodontics II, Affiliated Stomatological Hospital of Zunyi Medical University, Zunyi, 563000, China.
Xiaoyan GuanDepartment of Orthodontics II, Affiliated Stomatological Hospital of Zunyi Medical University, Zunyi, 563000, China. 1278279125@qq.com.
Jianguo LiuGuiZhou University Medical College, Guiyang, 550025, Guizhou Province, China. 13087891001@163.com.

Funding

The National Natural Science Foundation of China 82060205Zunyi Science and Technology Plan Project Zun Shi Ke He HZ Zi (2022) 423Zunyi Science and Technology Plan Project Zun Shi Ke He HZ Zi [2023] 82
6 · The paper itself

Abstract

Metformin has shown outstanding anti-inflammatory and osteogenic abilities. Mesenchymal stem cell-derived extracellular vesicles (EVs) reveal promising therapeutic potency by carrying various biomolecules. This study explored the effects of metformin on the therapeutic potential of EVs derived from human periodontal ligament stem cells (PDLSCs) for periodontitis. PDLSCs were cultured in osteogenic medium with or without metformin, and the supernatant was then collected separately to extract EVs and metformin-treated EVs (M-EVs). After identifying the characteristics, we evaluated the anti-inflammatory and osteogenic effects of EVs and M-EVs in vivo and in vitro. Osteogenic differentiation of PDLSCs was markedly enhanced after metformin treatment, and the effect was dramatically inhibited by GW4896, an inhibitor of EVs' secretion. Metformin significantly increased EVs' yields and improved their effects on cell proliferation, migration, and osteogenic differentiation. Moreover, metformin significantly enhanced the osteogenic ability of EVs on inflammatory PDLSCs. Animal experiments revealed that alveolar bone resorption was dramatically reduced in the EVs and M-EVs groups when compared to the periodontitis group, while the M-EVs group showed the lowest levels of alveolar bone loss. Metformin promoted the osteogenic differentiation of PDLSCs partly through EVs pathway and significantly enhanced the secretion of PDLSCs-EVs with superior pro-osteogenic and anti-inflammatory potential, thus improving EVs' therapeutic potential on periodontitis.

Indexed as

Cell DifferentiationExtracellular VesiclesMetforminOsteogenesisPeriodontal LigamentPeriodontitisStem CellsAlveolar Bone LossAnimalsCell MovementCell ProliferationCells, CulturedHumansMaleMesenchymal Stem CellsMiceMetforminAlveolar bone resorptionExtracellular VesiclesMetforminPDLSCsPeriodontitis

Identifiers

PMID39198490
PMCPMC11358454

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.