Evidence mapPaperPMID 39203366Full record

ArticleMicroorganisms2024

Comparing the Microbiome of the Adenoids in Children with Secretory Otitis Media and Children without Middle Ear Effusion.

Oļegs Sokolovs-Karijs, Monta Brīvība, Rihards Saksis, Maija Rozenberga, Laura Bunka, Francesca Girotto, Jana Osīte, Aigars Reinis, Gunta Sumeraga, Angelika Krūmiņa

Abstract read
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Article in Microorganisms, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Oļegs Sokolovs-KarijsDepartment of Otolaryngology, Riga Stradiņš University, 16 Dzirciema Street, LV-1007 Riga, Latvia.ORCID 0000-0002-7146-1803
Monta BrīvībaLatvian Biomedicine Research and Study Center, 1 Ratsupites Street, LV-1067 Riga, Latvia.
Rihards SaksisLatvian Biomedicine Research and Study Center, 1 Ratsupites Street, LV-1067 Riga, Latvia.ORCID 0009-0002-5017-5688
Maija RozenbergaLatvian Biomedicine Research and Study Center, 1 Ratsupites Street, LV-1067 Riga, Latvia.ORCID 0009-0004-7735-682X
Laura BunkaLatvian Biomedicine Research and Study Center, 1 Ratsupites Street, LV-1067 Riga, Latvia.
Francesca GirottoFaculty of Medicine, Riga Stradiņš University, 16 Dzirciema Street, LV-1007 Riga, Latvia.ORCID 0009-0000-7557-3477
Jana Osīte"Centrālā Laboratorrija", 1b. Šarlotes Street, LV-1011 Riga, Latvia.
Aigars ReinisDepartment of Biology and Microbiology, Riga Stradiņš University, 16 Dzirciema Street, LV-1007 Riga, Latvia.ORCID 0000-0002-3671-1213
Gunta SumeragaDepartment of Otolaryngology, Riga Stradiņš University, 16 Dzirciema Street, LV-1007 Riga, Latvia.ORCID 0000-0002-2781-8991
Angelika KrūmiņaDepartment of Infectology, Riga Stradiņš University, 16 Dzirciema Street, LV-1007 Riga, Latvia.

Funding

Latvian university No.8.2.2.0/20/I/006Riga Stradiņš university Nr. 6-DN-20/1/2024
6 · The paper itself

Abstract

backgroundThe adenoids, primary sites of microbial colonization in the upper airways, can influence the development of various conditions, including otitis media with effusion (OME). Alterations in the adenoid microbiota have been implicated in the pathogenesis of such conditions.

aimThis study aims to utilize 16S rRNA genetic sequencing to identify and compare the bacterial communities on the adenoid surfaces of children with OME and children with healthy middle ears. Additionally, we seek to assess the differences in bacterial diversity between these two groups. MATERIALS AND

methodsWe collected adenoid surface swabs from forty children, divided into two groups: twenty samples from children with healthy middle ears and twenty samples from children with OME. The V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified and sequenced using the Illumina MiSeq platform. Alpha and beta diversity indices were calculated, and statistical analyses were performed to identify significant differences in bacterial composition.

resultsAlpha diversity analysis, using Pielou's index, revealed significantly greater evenness in the bacterial communities on the adenoid surfaces of the healthy ear group compared with the OME group. Beta diversity analysis indicated greater variability in the microbial composition of the OME group. The most common bacterial genera in both groups were

conclusionOur study identified significant differences in the bacterial composition and diversity on the adenoid surfaces of children with healthy middle ears and those with OME. The OME group exhibited greater microbial variability and higher abundances of specific bacterial genera. These findings suggest that the adenoid surface microbiota may play a role in the pathogenesis of OME. Further research with larger sample sizes and control groups is needed to validate these results and explore potential clinical applications.

Indexed as

16S rRNA genetic sequencingadenoidsbacteriamicrobiomeotitis media with effusion

Identifiers

PMID39203366
PMCPMC11356538

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.