Evidence map›Paper›PMID 39208278›Full record

ArticleACS nano2024

Integrating Vascular Phenotypic and Proteomic Analysis in an Open Microfluidic Platform.

Sangmin Jung, Sunghun Cheong, Yoonho Lee, Jungseub Lee, Jihye Lee, Min-Seok Kwon, Young Sun Oh, Taewan Kim, Sungjae Ha, Sung Jae Kim and 3 more

Erratum issuedAbstract read
In one paragraph

Article in ACS nano, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Sangmin JungDepartment of Mechanical Engineering, Seoul National University, Seoul 08826, Republic of Korea.
Sunghun CheongInterdisciplinary Program in Bioengineering, Seoul National University, Seoul 08826, Republic of Korea.ORCID 0009-0007-8143-8432
Yoonho LeeInterdisciplinary Program in Bioengineering, Seoul National University, Seoul 08826, Republic of Korea.ORCID 0009-0005-6512-7939
Jungseub LeeDepartment of Mechanical Engineering, Seoul National University, Seoul 08826, Republic of Korea.
Jihye LeeTarget Link Therapeutics, Inc., Seoul 04545, Republic of Korea.
Min-Seok KwonTarget Link Therapeutics, Inc., Seoul 04545, Republic of Korea.
Young Sun OhDepartment of Mechanical Engineering, Seoul National University, Seoul 08826, Republic of Korea.
Taewan KimDepartment of Electrical and Computer Engineering, Seoul National University, Seoul 08826, Republic of Korea.
Sungjae HaProvaLabs, Inc., Seoul 08826, Republic of Korea.
Sung Jae KimDepartment of Electrical and Computer Engineering, Seoul National University, Seoul 08826, Republic of Korea.ORCID 0000-0002-8622-1765
Dong Hyun JoDepartment of Anatomy and Cell Biology, Seoul National University College of Medicine, Seoul 03080, Republic of Korea.ORCID 0000-0002-6320-6829
Jihoon KoDepartment of BioNano Technology, Gachon University, Seongnam-si, Gyeonggi-do 13120, Republic of Korea.
Noo Li JeonDepartment of Mechanical Engineering, Seoul National University, Seoul 08826, Republic of Korea.ORCID 0000-0003-0490-3592

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This research introduces a vascular phenotypic and proteomic analysis (VPT) platform designed to perform high-throughput experiments on vascular development. The VPT platform utilizes an open-channel configuration that facilitates angiogenesis by precise alignment of endothelial cells, allowing for a 3D morphological examination and protein analysis. We study the effects of antiangiogenic agents─bevacizumab, ramucirumab, cabozantinib, regorafenib, wortmannin, chloroquine, and paclitaxel─on cytoskeletal integrity and angiogenic sprouting, observing an approximately 50% reduction in sprouting at higher drug concentrations. Precise LC-MS/MS analyses reveal global protein expression changes in response to four of these drugs, providing insights into the signaling pathways related to the cell cycle, cytoskeleton, cellular senescence, and angiogenesis. Our findings emphasize the intricate relationship between cytoskeletal alterations and angiogenic responses, underlining the significance of integrating morphological and proteomic data for a comprehensive understanding of angiogenesis. The VPT platform not only advances our understanding of drug impacts on vascular biology but also offers a versatile tool for analyzing proteome and morphological features across various models beyond blood vessels.

Indexed as

Angiogenesis InhibitorsHuman Umbilical Vein Endothelial CellsProteomicsHumansNeovascularization, PhysiologicPhenotypeAngiogenesis Inhibitorsangiogenesisdrug screeningimage analysismicrophysiological systemopen microfluidicsproteomics

Identifiers

PMID39208278
PMCPMC11394367

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.