Evidence map›Paper›PMID 39209459›Full record

ArticleJournal of psychiatry & neuroscience : JPN

Plasma exosomes carrying mmu-miR-146a-5p and Notch signalling pathway-mediated synaptic activity in schizophrenia.

Zhichao Wang, Tong Wu, Houjia Hu, Alabed Ali A Alabed, Guangcheng Cui, Lei Sun, Zhenghai Sun, Yuchen Wang, Ping Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of psychiatry & neuroscience : JPN. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zhichao WangFrom the Departments of Academic Research, Qiqihar Medical University, Qiqihar, PR China (Z. Wang); the School of Basic Medical Sciences, Nanchang University, Nanchang, PR China (Hu); the Community Medicine Department, Faculty of Medicine, Lincoln University College, Malaysia (Alabed); the Department of Psychology, Qiqihar Medical University, Qiqihar, PR China (Wu, Cui, L. Sun, Z. Sun).
Tong WuFrom the Departments of Academic Research, Qiqihar Medical University, Qiqihar, PR China (Z. Wang); the School of Basic Medical Sciences, Nanchang University, Nanchang, PR China (Hu); the Community Medicine Department, Faculty of Medicine, Lincoln University College, Malaysia (Alabed); the Department of Psychology, Qiqihar Medical University, Qiqihar, PR China (Wu, Cui, L. Sun, Z. Sun).
Houjia HuFrom the Departments of Academic Research, Qiqihar Medical University, Qiqihar, PR China (Z. Wang); the School of Basic Medical Sciences, Nanchang University, Nanchang, PR China (Hu); the Community Medicine Department, Faculty of Medicine, Lincoln University College, Malaysia (Alabed); the Department of Psychology, Qiqihar Medical University, Qiqihar, PR China (Wu, Cui, L. Sun, Z. Sun).
Alabed Ali A AlabedFrom the Departments of Academic Research, Qiqihar Medical University, Qiqihar, PR China (Z. Wang); the School of Basic Medical Sciences, Nanchang University, Nanchang, PR China (Hu); the Community Medicine Department, Faculty of Medicine, Lincoln University College, Malaysia (Alabed); the Department of Psychology, Qiqihar Medical University, Qiqihar, PR China (Wu, Cui, L. Sun, Z. Sun).
Guangcheng CuiFrom the Departments of Academic Research, Qiqihar Medical University, Qiqihar, PR China (Z. Wang); the School of Basic Medical Sciences, Nanchang University, Nanchang, PR China (Hu); the Community Medicine Department, Faculty of Medicine, Lincoln University College, Malaysia (Alabed); the Department of Psychology, Qiqihar Medical University, Qiqihar, PR China (Wu, Cui, L. Sun, Z. Sun) gccui@qmu.edu.cn qyliping@qmu.edu.cn.
Lei SunFrom the Departments of Academic Research, Qiqihar Medical University, Qiqihar, PR China (Z. Wang); the School of Basic Medical Sciences, Nanchang University, Nanchang, PR China (Hu); the Community Medicine Department, Faculty of Medicine, Lincoln University College, Malaysia (Alabed); the Department of Psychology, Qiqihar Medical University, Qiqihar, PR China (Wu, Cui, L. Sun, Z. Sun).
Zhenghai SunFrom the Departments of Academic Research, Qiqihar Medical University, Qiqihar, PR China (Z. Wang); the School of Basic Medical Sciences, Nanchang University, Nanchang, PR China (Hu); the Community Medicine Department, Faculty of Medicine, Lincoln University College, Malaysia (Alabed); the Department of Psychology, Qiqihar Medical University, Qiqihar, PR China (Wu, Cui, L. Sun, Z. Sun).
Yuchen WangFrom the Departments of Academic Research, Qiqihar Medical University, Qiqihar, PR China (Z. Wang); the School of Basic Medical Sciences, Nanchang University, Nanchang, PR China (Hu); the Community Medicine Department, Faculty of Medicine, Lincoln University College, Malaysia (Alabed); the Department of Psychology, Qiqihar Medical University, Qiqihar, PR China (Wu, Cui, L. Sun, Z. Sun).
Ping LiFrom the Departments of Academic Research, Qiqihar Medical University, Qiqihar, PR China (Z. Wang); the School of Basic Medical Sciences, Nanchang University, Nanchang, PR China (Hu); the Community Medicine Department, Faculty of Medicine, Lincoln University College, Malaysia (Alabed); the Department of Psychology, Qiqihar Medical University, Qiqihar, PR China (Wu, Cui, L. Sun, Z. Sun).

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSchizophrenia is characterized by a complex interplay of genetic and environmental factors, leading to alterations in various molecular pathways that may contribute to its pathogenesis. Recent studies have shown that exosomal microRNAs could play essential roles in various brain disorders; thus, we sought to explore the potential molecular mechanisms through which microRNAs in plasma exosomes are involved in schizophrenia.

methodsWe obtained sequencing data sets (SUB12404730, SUB12422862, and SUB12421357) and transcriptome sequencing data sets (GSE111708, GSE108925, and GSE18981) from mouse models of schizophrenia using the Sequence Read Archive and the Gene Expression Omnibus databases, respectively. We performed differential expression analysis on mRNA to identify differentially expressed genes. We conducted Gene Ontology (GO) functional and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses to determine differentially expressed genes. Subsequently, we determined the intersection of differentially expressed microRNAs in plasma exosomes and in prefrontal cortex tissue. We retrieved downstream target genes of mmu-miR-146a-5p from TargetScan and used Cytoscape to visualize and map the microRNA-target gene regulatory network. We conducted in vivo experiments using MK-801-induced mouse schizophrenia models and in vitro experiments using cultured mouse neurons. The role of plasma exosomal miR-146a-5p in schizophrenia was validated using a cell counting kit, detection of lactate dehydrogenase, dual-luciferase assay, quantitative reverse transcription polymerase chain reaction, and Western blot analysis.

resultsDifferential genes were mainly enriched in synaptic regulation-related functions and pathways and were associated with neuronal degeneration. We found that mmu-miR-146a-5p was highly expressed in both prefrontal cortical tissue and plasma exosomes, which may be transferred to lobe cortical vertebral neurons, leading to the synergistic dysregulation of gene network functions and, therefore, promoting schizophrenia development. We found that mmu-miR-146a-5p may inhibit the Notch signalling pathway-mediated synaptic activity of mouse pyramidal neurons in the lobe cortex by targeting LIMITATIONS: The study's findings are based on animal models and in vitro experiments, which may not fully replicate the complexity of human schizophrenia.

conclusionOur findings suggest that mmu-miR-146a-5p in plasma-derived exosomes may play an important role in the pathogenesis of schizophrenia. Our results provide new insights into the underlying molecular mechanisms of the disease.

Indexed as

Disease Models, AnimalExosomesMice, Inbred C57BLMicroRNAsPrefrontal CortexSchizophreniaSignal TransductionAnimalsDizocilpine MaleateGene Regulatory NetworksMiceNeuronsReceptors, NotchSynapsesDizocilpine MaleateMicroRNAsMirn146 microRNA, mouseReceptors, Notch

Identifiers

PMID39209459
PMCPMC11374447

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.