Evidence map›Paper›PMID 39209986›Full record

ArticleScientific reports2024

CAPZB mRNA is a novel biomarker for cervical high-grade squamous lesions.

Xia Cai, Wanqiu Huang, Jian Huang, Xiuxiang Zhu, Lifeng Wang, Ziyin Xia, Ling Xu

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PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Xia CaiDepartment of Gynaecology, Minhang Hospital, Fudan University, Shanghai, 201199, China.
Wanqiu HuangKey Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Centre for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, 200240, China.
Jian HuangKey Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Centre for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, 200240, China.
Xiuxiang ZhuDepartment of Gynaecology, Minhang Hospital, Fudan University, Shanghai, 201199, China.
Lifeng WangDepartment of Gynaecology, Minhang Hospital, Fudan University, Shanghai, 201199, China.
Ziyin XiaDepartment of Gynaecology, Minhang Hospital, Fudan University, Shanghai, 201199, China.
Ling XuDepartment of Gynaecology, Minhang Hospital, Fudan University, Shanghai, 201199, China. xu_ling@fudan.edu.cn.

Funding

Hospital-level disciplines-Chronic disease Group-Cervical Diagnosis and Treatment Centre and obstetrics Endocrinology YJXK-2021-11
6 · The paper itself

Abstract

This study aimed to evaluate the potential of capping protein (actin filament) muscle Z-line subunit β (CAPZB) messenger ribonucleic acid (mRNA) levels as a biomarker for distinguishing low-grade squamous intraepithelial lesions of the cervix (LSIL) from high-grade squamous intraepithelial lesions of the cervix (HSIL). We collected a total of 166 cervical exfoliated cells and divided them into five groups based on histopathological results. Each sample was divided into two portions, one for fluorescence in situ hybridization (FISH) detection and the other for bisulfite sequencing polymerase chain reaction (BSP) detection. We found that FISH detection of CAPZB mRNA mean fluorescence intensity (MFI) and BSP detection of CAPZB deoxyribonucleic acid (DNA) percentage of methylation rate (PMR) performed as biomarkers for distinguishing HSIL from LSIL, with an area under the receiver operating characteristic curve (AUC), sensitivity, specificity and cut-off value of 0.893, 81.25%, 80.39% and 0.616, 0.794, 64.06%, 81.37% and 0.454, respectively. Furthermore, FISH detection of CAPZB mRNA exhibited a greater AUC (0.893) for the detection of HSIL than the CAPZB DNA methylation method (0.794), indicating the CAPZB mRNA levels can be used as a biomarker for assessing cervical lesions.

Indexed as

Biomarkers, TumorDNA MethylationIn Situ Hybridization, FluorescenceRNA, MessengerUterine Cervical NeoplasmsAdultCapZ Actin Capping ProteinFemaleHumansMiddle AgedROC CurveSquamous Intraepithelial Lesions of the CervixBiomarkers, TumorCapZ Actin Capping ProteinRNA, MessengerCAPZBCervical cancerDNA methylationFluorescence in situ hybridizationMessenger RNA

Identifiers

PMID39209986
PMCPMC11362286

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.