Evidence map›Paper›PMID 39215271›Full record

ArticleCellular & molecular biology letters2024

Single-cell RNA sequencing identifies inherent abnormalities of adipose-derived stem cells from nonlesional sites of patients with localized scleroderma.

Xuanyu Liu, Zhujun Li, Hayson Chenyu Wang, Meng Yuan, Jie Chen, Jiuzuo Huang, Nanze Yu, Zhou Zhou, Xiao Long

Abstract read
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In one paragraph

Article in Cellular & molecular biology letters, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Xuanyu Liu *State Key Laboratory of Cardiovascular Disease, Beijing Key Laboratory for Molecular Diagnostics of Cardiovascular Diseases, Center of Laboratory Medicine, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, China.
Zhujun Li *Department of Plastic Surgery, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, 100730, China.
Hayson Chenyu WangDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
Meng YuanState Key Laboratory of Cardiovascular Disease, Beijing Key Laboratory for Molecular Diagnostics of Cardiovascular Diseases, Center of Laboratory Medicine, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, China.
Jie ChenDepartment of Plastic Surgery, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, 100730, China.
Jiuzuo HuangDepartment of Plastic Surgery, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, 100730, China.
Nanze YuDepartment of Plastic Surgery, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, 100730, China.
Zhou ZhouState Key Laboratory of Cardiovascular Disease, Beijing Key Laboratory for Molecular Diagnostics of Cardiovascular Diseases, Center of Laboratory Medicine, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, China. zhouzhou@fuwaihospital.org.ORCID http://orcid.org/0000-0002-1060-0355
Xiao LongDepartment of Plastic Surgery, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, 100730, China. pumclongxiao@126.com.

Funding

the CAMS Innovation Fund for Medical Sciences 2021-I2M-1-008the National High Level Hospital Clinical Research Funding 2022-PUMCH-A-210the National High Level Hospital Clinical Research Funding 2022-PUMCH-C-025the National High Level Hospital Clinical Research Funding 2023-GSP-RC-21
6 · The paper itself

Abstract

backgroundLocalized scleroderma (LoS) is an autoimmune disorder that primarily affects the skin, and is often treated with autologous fat grafting (AFG). Nevertheless, the retention rate of AFG in patients with LoS is typically low. We hypothesize that the low retention rate may be partially attributed to the inherent abnormalities of adipose-derived stem cells (ASCs) from nonlesional sites of patients with LoS.

methodsWe performed a comparative analysis of the single-cell transcriptome of the SVF from nonlesional sites of patients with LoS and healthy donors, including cellular compositional analysis, differential expression analysis, and high-dimensional weighted gene coexpression network analysis. Experimental validation with fluorescence-activated cell sorting and bleomycin-induced skin fibrosis mice models were conducted.

resultsWe found a significant reduction in the relative proportion of CD55

conclusionsWe found that the low retention rate of AFG may be partially ascribed to the reduced pool of interstitial progenitor cells (CD55

Indexed as

Adipose TissueScleroderma, LocalizedSingle-Cell AnalysisStem CellsAdultAnimalsDisease Models, AnimalFemaleFibrosisHumansMaleMiceMice, Inbred C57BLMiddle AgedSequence Analysis, RNASkinAdipose-derived stem cellsCD55Localized sclerodermaSingle-cell RNA sequencingStromal vascular fraction

Identifiers

PMID39215271
PMCPMC11363359

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.