Evidence map›Paper›PMID 39217195›Full record

ArticleBritish journal of cancer2024

Altered expression of vesicular trafficking machinery in prostate cancer affects lysosomal dynamics and provides insight into the underlying biology and disease progression.

Bukuru D Nturubika, Carlos M Guardia, David C Gershlick, Jessica M Logan, Carmela Martini, Jessica K Heatlie, Joanna Lazniewska, Courtney Moore, Giang T Lam, Ka L Li and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in British journal of cancer, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
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  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Bukuru D NturubikaMechanisms in Cell Biology and Diseases Research Group, Clinical and Health Sciences, University of South Australia, Adelaide, SA, 5000, Australia. bukuru.nturubika@mymail.unisa.edu.au.ORCID http://orcid.org/0000-0002-3664-9771
Carlos M GuardiaPlacental Cell Biology Group, National Institute of Environmental Health and Science, National Institutes of Health, Research Triangle Park, NC, 27709, USA.
David C GershlickCambridge Institute for Medical Research, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0002-0602-210X
Jessica M LoganMechanisms in Cell Biology and Diseases Research Group, Clinical and Health Sciences, University of South Australia, Adelaide, SA, 5000, Australia.
Carmela MartiniMechanisms in Cell Biology and Diseases Research Group, Clinical and Health Sciences, University of South Australia, Adelaide, SA, 5000, Australia.
Jessica K HeatlieMechanisms in Cell Biology and Diseases Research Group, Clinical and Health Sciences, University of South Australia, Adelaide, SA, 5000, Australia.
Joanna LazniewskaMechanisms in Cell Biology and Diseases Research Group, Clinical and Health Sciences, University of South Australia, Adelaide, SA, 5000, Australia.
Courtney MooreMechanisms in Cell Biology and Diseases Research Group, Clinical and Health Sciences, University of South Australia, Adelaide, SA, 5000, Australia.
Giang T LamMechanisms in Cell Biology and Diseases Research Group, Clinical and Health Sciences, University of South Australia, Adelaide, SA, 5000, Australia.ORCID http://orcid.org/0000-0002-2416-1779
Ka L LiMechanisms in Cell Biology and Diseases Research Group, Clinical and Health Sciences, University of South Australia, Adelaide, SA, 5000, Australia.
Ben S-Y UngQuality Use of Medicines and Pharmacy Research Centre, University of South Australia City East Campus, Frome Rd, Adelaide, SA, 5000, Australia.ORCID http://orcid.org/0000-0001-7667-3648
Robert D BrooksMechanisms in Cell Biology and Diseases Research Group, Clinical and Health Sciences, University of South Australia, Adelaide, SA, 5000, Australia.
Shane M HickeyMechanisms in Cell Biology and Diseases Research Group, Clinical and Health Sciences, University of South Australia, Adelaide, SA, 5000, Australia.
Andrew G BertCentre for Cancer Biology, University of South Australia, Adelaide, SA, 5000, Australia.
Philip A GregoryCentre for Cancer Biology, University of South Australia, Adelaide, SA, 5000, Australia.
Lisa M ButlerSouth Australian ImmunoGENomics Cancer Institute and Freemasons Centre for Male Health and Wellbeing, University of Adelaide, Adelaide, SA, 5000, Australia.ORCID http://orcid.org/0000-0003-2698-3220
John J O'LearyDepartment of Histopathology, Trinity College Dublin, Dublin, Dublin 8, Ireland.
Douglas A Brooks *Mechanisms in Cell Biology and Diseases Research Group, Clinical and Health Sciences, University of South Australia, Adelaide, SA, 5000, Australia. doug.brooks@unisa.edu.au.
Ian R D Johnson *Mechanisms in Cell Biology and Diseases Research Group, Clinical and Health Sciences, University of South Australia, Adelaide, SA, 5000, Australia.ORCID http://orcid.org/0000-0001-7875-3510

Funding

Autophagy and Intracellular Trafficking in Placental BiologyZIAES103370 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI GUARDIA, CARLOS · 2022 to 2025
$9.2M
Department of Health | National Health and Medical Research Council (NHMRC) APP1092904Intramural NIH HHS ZIA ES103370U.S. Department of Health & Human Services | NIH | National Institute of Environmental Health Sciences (NIEHS) ZIA ES103370-01
6 · The paper itself

Abstract

backgroundThis study focuses on the role of lysosomal trafficking in prostate cancer, given the essential role of lysosomes in cellular homoeostasis.

methodsLysosomal motility was evaluated using confocal laser scanning microscopy of LAMP-1-transfected prostate cells and spot-tracking analysis. Expression of lysosomal trafficking machinery was evaluated in patient cohort databases and through immunohistochemistry on tumour samples. The roles of vesicular trafficking machinery were evaluated through over-expression and siRNA. The effects of R1881 treatment on lysosome vesicular trafficking was evaluated by RNA sequencing, protein quantification and fixed- and live-cell microscopy.

resultsAltered regulation of lysosomal trafficking genes/proteins was observed in prostate cancer tissue, with significant correlations for co-expression of vesicular trafficking machinery in Gleason patterns. The expression of trafficking machinery was associated with poorer patient outcomes. R1881 treatment induced changes in lysosomal distribution, number, and expression of lysosomal vesicular trafficking machinery in hormone-sensitive prostate cancer cells. Manipulation of genes involved in lysosomal trafficking events induced changes in lysosome positioning and cell phenotype, as well as differential effects on cell migration, in non-malignant and prostate cancer cells.

conclusionsThese findings provide novel insights into the altered regulation and functional impact of lysosomal vesicular trafficking in prostate cancer pathogenesis.

Indexed as

Disease ProgressionLysosomesProstatic NeoplasmsCell Line, TumorCell MovementGene Expression Regulation, NeoplasticHumansMaleProtein Transport

Identifiers

PMID39217195
PMCPMC11473802

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.