Evidence map›Paper›PMID 39229161›Full record

ArticlebioRxiv : the preprint server for biology2024

Transmembrane channel-like 4 and 5 proteins at microvillar tips are potential ion channels and lipid scramblases.

Seham Ebrahim, Angela Ballesteros, W Sharon Zheng, Shounak Mukherjee, Gaizun Hu, Wei-Hsiang Weng, Jonathan S Montgomery, Yaw Agyemang, Runjia Cui, Willy Sun and 4 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Seham EbrahimCenter for Membrane and Cell Physiology, Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22903, USA.ORCID 0000-0002-6762-2432
Angela BallesterosLaboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA.ORCID 0000-0001-5796-6875
W Sharon ZhengCenter for Membrane and Cell Physiology, Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22903, USA.
Shounak MukherjeeDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
Gaizun HuCenter for Membrane and Cell Physiology, Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22903, USA.
Wei-Hsiang WengDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
Jonathan S MontgomeryDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
Yaw AgyemangDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
Runjia CuiLaboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA.
Willy SunLaboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA.
Evan KrystofiakLaboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA.
Mark P FosterDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
Marcos SotomayorDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
Bechara KacharLaboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD 20892, USA.

Funding

Advanced Imaging CoreZICDC000081 · NIDCD · NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS · PI WINKLER, DENNIS · 2012 to 2025
$17.4M
MOLECULAR BASIS OF TRANSDUCTION IN AUDITORY SENSORY ORGANSZ01DC000002 · NIDCD · NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS · PI KACHAR, BECHARA · 1989 to 2008
$3.9M
Molecular biophysics predoctoral training at the Ohio State UniversityT32GM144293 · NIGMS · OHIO STATE UNIVERSITY · PI CHARLES E BELL, Ralf A Bundschuh · 2022 to 2026
$1.1M
Intramural NIH HHS Z01 DC000002Intramural NIH HHS ZIC DC000081NIGMS NIH HHS T32 GM144293
6 · The paper itself

Abstract

Microvilli-membrane bound actin protrusions on the surface of epithelial cells-are sites of critical processes including absorption, secretion, and adhesion. Increasing evidence suggests microvilli are mechanosensitive, but underlying molecules and mechanisms remain unknown. Here, we localize transmembrane channel-like proteins 4 and 5 (TMC4 and 5) and calcium and integrin binding protein 3 (CIB3) to microvillar tips in intestinal epithelial cells, near glycocalyx insertion sites. We find that TMC5 colocalizes with CIB3 in cultured cells and that a TMC5 fragment forms a complex with CIB3

Identifiers

PMID39229161
PMCPMC11370596

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.