Evidence map›Paper›PMID 39229992›Full record

ArticleCombinatorial chemistry & high throughput screening2025

Zuogui Pills Improve the Learning and Memory Ability of Brain-Aging Mice through the Estrogen Receptor ERα Methylation Pathway.

Yang Huang, Lili Zhong, Jing Yang, Qiaomei Dai, Li Zhao, Mengdi Zhang, Hong Liu

Abstract read
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In one paragraph

Article in Combinatorial chemistry & high throughput screening, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Yang HuangGraduate School, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China.
Lili ZhongDepartment of Pathology, The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China.
Jing YangDepartment of Pathology and Pathophysiology, School of Basic Medical Sciences, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China.
Qiaomei DaiDepartment of Pathology and Pathophysiology, School of Basic Medical Sciences, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China.
Li ZhaoGraduate School, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China.
Mengdi ZhangGraduate School, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China.
Hong LiuDepartment of Pathology and Pathophysiology, School of Basic Medical Sciences, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China.

Funding

Joint Guidance Project of Heilongjiang Natural Science Foundation LH2022H068Postdoctoral Foundation of Heilongjiang Province LBHZ15207Postdoctoral Science Foundation of China 2016M591565Postgraduate Innovative Research Project Initiation Fund of Heilongjiang University of Chinese Medicine 2023yjscx020Project for Young Reserve Talents of Harbin Science and Technology Bureau 2016RAQXJ202Science and Technology Plan of Heilongjiang Provincial Health Commission 202202080996Science Foundation for Youths of Heilongjiang Province QC2015102Surface Project of the National Natural Science Foundation of China 82074288Youth Fund Project of the National Natural Science Foundation of China 81403288
6 · The paper itself

Abstract

introductionSex hormones are important factors in maintaining brain function and acting as brain protectors. Recent research suggests that neuronal damage in brain aging may be linked to the methylation of the estrogen receptor α (ERα). However, the mechanism of Zuogui Pills (ZGW) in brain-aging ERα DNA methylation and neuronal repair remains unknown.

methodsD-galactose-induced ovary removal mice were used as a model of aging. Changes in estrous cycle were detected in mice by vaginal cell smear. Animal behavior tests, including the Morris water maze (MWM) and new object recognition (NOR) test, were conducted. Hematoxylin- eosin (HE) and Nissl-staining were carried out to assess hippocampal neurogenesis. Enzymelinked immunosorbent assay (ELISA) was performed for 5-methylcytosine methylation levels, and immunohistochemistry (IHC) and western blotting (WB) experiments were performed to assess ERα/DNA methyltransferase 1 (DNMT1) expression after ZGW treatment. Finally, bisulfite sequencing PCR (BSP) analysis was performed to identify methylated differentially expressed estrogen receptor 1 (ESR1) gene in D-gal-induced senescent neurons before and after ZGW treatment.

resultsWe found that ERα methylation was involved in the delayed brain ageing process of ZGW. Mechanistically, ZGW can improve the learning and memory ability of brain-aging mice, reduce the expression of 5-methylcytosine (5-mc) in serum, increase the amount of ERα, inhibit the expression of DNMT1, and significantly reduce methylated expression of the ESRI gene.

conclusionOur data suggested that ZGW slowed down D-gal-induced brain aging in mice, and these results showed that ZGW is beneficial for aging. It may be used for neuronal protection in aging.

Indexed as

AgingBrainDrugs, Chinese HerbalEstrogen Receptor alphaMemoryAnimalsDNA MethylationFemaleGalactoseMiceDrugs, Chinese HerbalEsr1 protein, mouseEstrogen Receptor alphaGalactoseanti-agingDNA methylationERαESRI gene.MemoryZuogui pill

Identifiers

PMID39229992

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.