Evidence map›Paper›PMID 39237579›Full record

ArticleScientific reports2024

IGF-I concentration determines cell fate by converting signaling dynamics as a bifurcation parameter in L6 myoblasts.

Ryosuke Okino, Kazuaki Mukai, Shunpei Oguri, Masato Masuda, Satoshi Watanabe, Yosuke Yoneyama, Sumine Nagaosa, Takafumi Miyamoto, Atsushi Mochizuki, Shin-Ichiro Takahashi and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Heparin-Modified Aligned Collagen Scaffolds Enhance In Vitro Myogenesis.Journal of biomedical materials research. Part A · 2026
    Article
  2. Heparin-modified aligned collagen scaffolds enhancebioRxiv : the preprint server for biology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Ryosuke Okino *Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Kazuaki Mukai *Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Shunpei OguriDepartment of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Masato MasudaDepartment of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Satoshi WatanabeAdvanced Institute for Materials Research, Tohoku University, Sendai, Japan.
Yosuke YoneyamaDepartment of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Sumine NagaosaDepartment of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Takafumi MiyamotoDepartment of Endocrinology and Metabolism, Institute of Medicine, University of Tsukuba, Ibaraki, Japan.
Atsushi MochizukiLaboratory of Mathematical Biology, Institute for Life and Medical Sciences, Kyoto University, Kyoto, Japan.
Shin-Ichiro TakahashiDepartment of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Fumihiko Hakuno *Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. hakuno@g.ecc.u-tokyo.ac.jp.

Funding

Bio-oriented Technology Research Advancement Institution Cross-ministerial Moonshot Agriculture, Forestry, and Fisheries Research and Development Program, "Technologies for Smart Bio-industry and Agriculture,"Japan Society for the Promotion of Science A16208028Japan Society for the Promotion of Science A22248030Japan Society for the Promotion of Science B15H04583Japan Society for the Promotion of Science B22H02528Japan Society for the Promotion of Science B24380152Japan Society for the Promotion of Science S25221204
6 · The paper itself

Abstract

Insulin-like growth factor (IGF)-I mediates long-term activities that determine cell fate, including cell proliferation and differentiation. This study aimed to characterize the mechanisms by which IGF-I determines cell fate from the aspect of IGF-I signaling dynamics. In L6 myoblasts, myogenic differentiation proceeded under low IGF-I levels, whereas proliferation was enhanced under high levels. Mathematical and experimental analyses revealed that IGF-I signaling oscillated at low IGF-I levels but remained constant at high levels, suggesting that differences in IGF-I signaling dynamics determine cell fate. We previously reported that differential insulin receptor substrate (IRS)-1 levels generate a driving force for cell competition. Computational simulations and immunofluorescence analyses revealed that asynchronous IRS-1 protein oscillations were synchronized during myogenic processes through cell competition. Disturbances of cell competition impaired signaling synchronization and cell fusion, indicating that synchronization of IGF-I signaling oscillation is critical for myoblast cell fusion to form multinucleate myotubes.

Indexed as

Cell DifferentiationInsulin-Like Growth Factor IMyoblastsSignal TransductionAnimalsCell FusionCell LineCell ProliferationInsulin Receptor Substrate ProteinsMuscle DevelopmentMuscle Fibers, SkeletalRatsInsulin-Like Growth Factor IInsulin Receptor Substrate ProteinsBifurcation analysisCell competitionInsulin-like growth factor-IL6 myoblastMyogenesisSignaling dynamicsSignaling oscillation

Identifiers

PMID39237579
PMCPMC11377782

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.