Evidence map›Paper›PMID 39240513›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Isolation, Culture, and Quality Assessment of Clinical-Grade Corneal Stromal Stem Cells.

Mithun Santra, Moira L Geary, Martha L Funderburgh, Gary H F Yam

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Mithun SantraCorneal Regeneration Laboratory, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Moira L GearyCorneal Regeneration Laboratory, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Martha L FunderburghCorneal Regeneration Laboratory, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Gary H F YamCorneal Regeneration Laboratory, Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. gary.yam@pitt.edu.

Funding

Virus Production and Manipulation of Protein/Gene Expression ModuleP30EY008098 · NEI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Ethan A Rossi · 1989 to 2026
$17.8M
Stem Cells for Corneal EngineeringR01EY016415 · NEI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI DU, YIQIN · 2006 to 2018
$4.5M
Cell Therapy Program with Scale-up cGMP Manufacturing of Human Corneal Stromal Stem CellsU01EY035252 · NEI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI SIKDER, DEVANJAN, YAM, HIN FAI · 2023 to 2024
$1.0M
NEI NIH HHS P30 EY008098NEI NIH HHS R01 EY016415NEI NIH HHS U01 EY035252
6 · The paper itself

Abstract

The challenge of treating corneal scarring through keratoplasties lies in the limited availability of donor tissue. Various studies have shown the therapeutic use of cultivated corneal stromal stem cells (CSSCs) to mitigate tissue inflammation and suppress fibrosis and scar tissue formation in preclinical corneal wound models. To develop CSSC therapy for clinical trials on patients with corneal scarring, it is necessary to generate clinical-grade CSSCs in compliant to Good Manufacturing Practice (GMP) regulations. This chapter elucidates human CSSC isolation, culture, and cryopreservation under GMP-compliant conditions. It underscores quality assessment encompassing morphological traits, expression of stemness markers, anti-inflammatory activity, and keratocyte differentiation potency.

Indexed as

Cell Culture TechniquesCell DifferentiationCorneal StromaBiomarkersCells, CulturedCell SeparationCryopreservationHumansStem CellsStromal CellsBiomarkersCell cultureCorneal opacitiesCorneal stromal stem cellsGood manufacturing practice (GMP)Quality assessment

Identifiers

PMID39240513
PMCPMC13270824

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.