Evidence mapPaperPMID 39247793Full record

ArticleDrug design, development and therapy2024

Semaglutide Alleviates Ovary Inflammation via the AMPK/SIRT1/NF‑κB Signaling Pathway in Polycystic Ovary Syndrome Mice.

Mei Liu, Sili Guo, Xiaohan Li, Yang Tian, Yanjie Yu, Lili Tang, Qimei Sun, Ting Zhang, Mingwei Fan, Lili Zhang and 5 more

Abstract read
In one paragraph

Article in Drug design, development and therapy, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed, 2 pooled it
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 2 syntheses or guidelines pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Mei Liu *Department of Endocrinology and Metabolism, Binzhou Medical University Hospital, Binzhou, Shandong, People's Republic of China.
Sili Guo *Department of Endocrinology and Metabolism, Binzhou Medical University Hospital, Binzhou, Shandong, People's Republic of China.
Xiaohan Li *Department of Endocrinology and Metabolism, Binzhou Medical University Hospital, Binzhou, Shandong, People's Republic of China.
Yang TianDepartment of Endocrinology and Metabolism, Binzhou Medical University Hospital, Binzhou, Shandong, People's Republic of China.
Yanjie YuDepartment of Ultrasound Medicine, Binzhou Medical University Hospital, Binzhou, Shandong, People's Republic of China.
Lili TangDepartment of Endocrinology and Metabolism, Binzhou Medical University Hospital, Binzhou, Shandong, People's Republic of China.
Qimei SunDepartment of Endocrinology and Metabolism, Binzhou Medical University Hospital, Binzhou, Shandong, People's Republic of China.
Ting ZhangDepartment of Endocrinology and Metabolism, Binzhou Medical University Hospital, Binzhou, Shandong, People's Republic of China.
Mingwei FanDepartment of Gastroenterology, Binzhou Medical University Hospital, Binzhou, Shandong, People's Republic of China.
Lili ZhangDepartment of Endocrinology and Metabolism, Binzhou Medical University Hospital, Binzhou, Shandong, People's Republic of China.
Yingjiang XuDepartment of Interventional Vascular Surgery, Binzhou Medical University Hospital, Binzhou, Shandong, People's Republic of China.
Jiajia AnDepartment of Clinical Laboratory, Binzhou Medical University Hospital, Binzhou, Shandong, People's Republic of China.
Xiangqian GaoDepartment of Pathology, Binzhou Medical University Hospital, Binzhou, Shandong, People's Republic of China.
Lei HanDepartment of Reproductive Medicine, Binzhou Medical University Hospital, Binzhou, Shandong, People's Republic of China.ORCID 0000-0001-8209-5813
Lei ZhangDepartment of Endocrinology and Metabolism, Binzhou Medical University Hospital, Binzhou, Shandong, People's Republic of China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: GLP-1 receptor agonists (GLP-1 RA) have been proven to treat several metabolic diseases; however, the effects of GLP-1 RA on polycystic ovary syndrome (PCOS) remain unclear. Here, we aimed to investigate whether semaglutide, a novel GLP-1 RA, could alleviate ovarian inflammation in PCOS mice. Methods: Female C57BL/6J mice were subcutaneously injected with dehydroepiandrosterone for 21 days to establish the PCOS model. Then the mice were randomly divided into three groups: PCOS group (n = 6), S-0.42 group (semaglutide 0.42 mg/kg/w, n = 6), and S-0.84 group (semaglutide 0.84 mg/kg/w, n = 6). The remaining six mice were used as controls (NC). After 28 days of intervention, serum sex hormones and inflammatory cytokine levels were measured. Hematoxylin and eosin staining was used to observe the ovarian morphology. Immunohistochemical staining was used to detect the relative expression of CYP19A1, TNF-α, IL-6, IL-1β, and NF-κB in ovaries. CYP17A1 and StAR were detected using immunofluorescence staining. Finally, the relative expressions of AMPK, pAMPK, SIRT1, NF-κB, IκBα, pIκBα, TNF-α, IL-6, and IL-1β were measured using Western blotting. Results: First, after intervention with semaglutide, the weight of the mice decreased, insulin resistance improved, and the estrous cycle returned to normal. Serum testosterone and IL-1β levels decreased significantly, whereas estradiol and progestin levels increased significantly. Follicular cystic dilation significantly improved. The expression of TNF-α, IL-6, IL-1β, NF-κB, CYP17A1, and StAR in the ovary was significantly downregulated, whereas CYP19A1 expression was upregulated after the intervention. Finally, we confirmed that semaglutide alleviates ovarian tissue inflammation and improves PCOS through the AMPK/SIRT1/NF-κB signaling pathway. Conclusion: Semaglutide alleviates ovarian inflammation via the AMPK/SIRT1/NF‑κB signaling pathway in PCOS mice.

Indexed as

Glucagon-Like Peptide-1 Receptor AgonistsGlucagon-Like PeptidesInflammationPolycystic Ovary SyndromeSignal TransductionAMP-Activated Protein KinasesAnimalsDisease Models, AnimalFemaleMiceMice, Inbred C57BLNF-kappa BOvarySemaglutideSirtuin 1AMP-Activated Protein KinasesGlucagon-Like Peptide-1 Receptor AgonistsGlucagon-Like PeptidesNF-kappa BSemaglutideSirt1 protein, mouseSirtuin 1GLP-1 receptor agonistinflammationpolycystic ovary syndromesemaglutide

Identifiers

PMID39247793
PMCPMC11380913

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.