Evidence map›Paper›PMID 39259408›Full record

ArticleCell biochemistry and biophysics2025

Endoplasmic Reticulum Stress in Myometrial Smooth Muscle Cells and Spontaneous Contraction Changes in the Uterus of Dehydroepiandrosterone-induced Polycystic Ovary Syndrome Rats.

Meryem Ilkay Karagul, Ayse Yildirim, Zehra Demiray Asoglu, Serdar Dogan, Savas Aktas, Ismail Un, Ibrahim Omer Barlas

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Article in Cell biochemistry and biophysics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Meryem Ilkay KaragulDepartment of Histology and Embryology, Faculty of Medicine, Hatay Mustafa Kemal University, Hatay, Turkey. ilkaykaragul@gmail.com.
Ayse YildirimDepartment of Histology and Embryology, Faculty of Medicine, Hatay Mustafa Kemal University, Hatay, Turkey.
Zehra Demiray AsogluDepartment of Histology and Embryology, Faculty of Medicine, Hatay Mustafa Kemal University, Hatay, Turkey.
Serdar DoganDepartment of Medical Biochemistry, Faculty of Medicine, Hatay Mustafa Kemal University, Hatay, Turkey.
Savas AktasDepartment of Histology and Embryology, Faculty of Medicine, Mersin University, Mersin, Turkey.
Ismail UnDepartment of Medical Pharmacology, Faculty of Medicine, Mersin University, Mersin, Turkey.
Ibrahim Omer BarlasDepartment of Medical Biology and Genetics, Faculty of Medicine, Mersin University, Mersin, Turkey.

Funding

Research Infrastructure Support Projects (ARADEP) of Hatay Mustafa Kemal University 22.A.005
6 · The paper itself

Abstract

Myometrial changes in polycystic ovary syndrome (PCOS) are poorly investigated. Thus, we aimed to investigate endoplasmic reticulum (ER) stress in myometrial smooth muscle cells and changes in spontaneous uterine contraction in PCOS. Twenty-one female Sprague-Dawley rats (21 days old) were divided into control (n = 7), vehicle (n = 7) and PCOS (n = 7) groups. While the control group was not injected subcutaneously, the vehicle group was injected subcutaneously with sesame oil (0.2 ml/day) for 20 consecutive days. The PCOS group was injected subcutaneously with dehydroepiandrosterone (6 mg/100 g/day dissolved in 0.2 ml sesame oil) for 20 consecutive days. Blood samples were collected for the measurement of follicle stimulating-hormone (FSH), luteinizing hormone (LH), testosterone (T), estradiol (E2) and glucose-regulated protein 78 (GRP78). The mRNA expression of GRP78 in the uterine tissue samples was analysed by quantitative real-time polymerase chain reaction. GRP78 protein expression was assessed by immunohistochemistry. Myometrial smooth muscle cells were examined by transmission electron microscopy. Uterine contractions were evaluated with isolated organ bath experiments. In the PCOS group, T and LH levels increased significantly, although FSH and E2 levels decreased, but this decrease was not statistically significant. Additionally, GRP78 levels increased significantly in the PCOS group. In the PCOS group, the mRNA level, immunostaining intensity of GRP78, and ER damage grade increased, but the uterine tissue calcium levels, and the frequency and amplitude of spontaneous uterine contractions decreased. The results indicated that increased ER stress in myometrial smooth muscle cells may play a causative role in the decreased spontaneous uterine contractions in PCOS.

Indexed as

DehydroepiandrosteroneEndoplasmic Reticulum StressMyocytes, Smooth MuscleMyometriumPolycystic Ovary SyndromeUterine ContractionUterusAnimalsEndoplasmic Reticulum Chaperone BiPEstradiolFemaleFollicle Stimulating HormoneHeat-Shock ProteinsLuteinizing HormoneRatsRats, Sprague-DawleyDehydroepiandrosteroneEndoplasmic Reticulum Chaperone BiPEstradiolFollicle Stimulating HormoneGRP78 protein, ratHeat-Shock ProteinsLuteinizing HormoneTestosteroneCalcium levelEndoplasmic reticulum stressGRP78Polycystic ovary syndromeUterine contraction

Identifiers

PMID39259408

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.