Evidence mapPaperPMID 39261583Full record

ArticleScientific reports2024

Alpha lipoic acid diminishes migration and invasion in hepatocellular carcinoma cells through an AMPK-p53 axis.

Florencia Hidalgo, Anabela C Ferretti, Carla Borini Etichetti, Emilia Baffo, Alejandro P Pariani, Tomás Rivabella Maknis, Javier Bussi, Javier E Girardini, María C Larocca, Cristián Favre

Abstract read
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Alpha-Lipoic Acid-Mediated Inhibition of LTBCurrent therapeutic research, clinical and experimental · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Florencia Hidalgo *Institute of Experimental Physiology (IFISE), School of Biochemical and Pharmaceutical Sciences, CONICET-University of Rosario, Suipacha 570, S2002LRL, Rosario, Argentina.
Anabela C Ferretti *Institute of Experimental Physiology (IFISE), School of Biochemical and Pharmaceutical Sciences, CONICET-University of Rosario, Suipacha 570, S2002LRL, Rosario, Argentina.
Carla Borini EtichettiInstitute of Clinical and Experimental Immunology of Rosario (IDICER), CONICET-University of Rosario, Rosario, Argentina.
Emilia BaffoInstitute of Experimental Physiology (IFISE), School of Biochemical and Pharmaceutical Sciences, CONICET-University of Rosario, Suipacha 570, S2002LRL, Rosario, Argentina.
Alejandro P ParianiInstitute of Experimental Physiology (IFISE), School of Biochemical and Pharmaceutical Sciences, CONICET-University of Rosario, Suipacha 570, S2002LRL, Rosario, Argentina.
Tomás Rivabella MaknisInstitute of Experimental Physiology (IFISE), School of Biochemical and Pharmaceutical Sciences, CONICET-University of Rosario, Suipacha 570, S2002LRL, Rosario, Argentina.
Javier BussiSchool of Statistics, University of Rosario, Rosario, Argentina.
Javier E GirardiniInstitute of Clinical and Experimental Immunology of Rosario (IDICER), CONICET-University of Rosario, Rosario, Argentina.
María C LaroccaInstitute of Experimental Physiology (IFISE), School of Biochemical and Pharmaceutical Sciences, CONICET-University of Rosario, Suipacha 570, S2002LRL, Rosario, Argentina.
Cristián FavreInstitute of Experimental Physiology (IFISE), School of Biochemical and Pharmaceutical Sciences, CONICET-University of Rosario, Suipacha 570, S2002LRL, Rosario, Argentina. cfavre@gmail.com.

Funding

Agencia Nacional de Promoción Científica y Tecnológica PICT 2021-427CONICET PIP 11220120100287CO
6 · The paper itself

Abstract

Hepatocellular carcinoma (HCC) associated with viral or metabolic liver diseases is a growing cancer without effective therapy. AMPK is downregulated in HCC and its activation diminishes tumor growth. Alpha lipoic acid (ALA), an indirect AMPK activator that inhibits hepatic steatosis, shows antitumor effects in different cancers. We aimed to study its putative action in liver-cancer derived cell lines through AMPK signaling. We performed cytometric studies for apoptosis and cell cycle, and 2D and 3D migration analysis in HepG2/C3A and Hep3B cells. ALA led to significant inhibition of cell migration/invasion only in HepG2/C3A cells. We showed that these effects depended on AMPK, and ALA also increased the levels and nuclear compartmentalization of the AMPK target p53. The anti-invasive effect of ALA was abrogated in stable-silenced (shTP53) versus isogenic-TP53 HepG2/C3A cells. Furthermore, ALA inhibited epithelial-mesenchymal transition (EMT) in control HepG2/C3A but not in shTP53 nor in Hep3B cells. Besides, we spotted that in patients from the HCC-TCGA dataset some EMT genes showed different expression patterns or survival depending on TP53. ALA emerges as a potent activator of AMPK-p53 axis in HCC cells, and it decreases migration/invasion by reducing EMT which could mitigate the disease in wild-type TP53 patients.

Indexed as

AMP-Activated Protein KinasesCarcinoma, HepatocellularCell MovementEpithelial-Mesenchymal TransitionLiver NeoplasmsThioctic AcidTumor Suppressor Protein p53ApoptosisCell Line, TumorGene Expression Regulation, NeoplasticHep G2 CellsHumansNeoplasm InvasivenessSignal TransductionAMP-Activated Protein KinasesThioctic AcidTP53 protein, humanTumor Suppressor Protein p53AMPKEMTHepatocarcinomaMigrationSignalingTP53

Identifiers

PMID39261583
PMCPMC11390941

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.